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Serpin F1 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Serpin F1 protein is part of the secreted Frizzled-related protein (sFRP) family and regulates the Wnt signaling pathway. As a member, Serpin F1 may interact with Wnt ligands and Frizzled receptors to regulate key cellular processes. Serpin F1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin F1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Serpin F1 Protein, Rat (HEK293, His)
  • Serpin F1 Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin F1 protein is part of the secreted Frizzled-related protein (sFRP) family and regulates the Wnt signaling pathway. As a member, Serpin F1 may interact with Wnt ligands and Frizzled receptors to regulate key cellular processes. Serpin F1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

Background

SFRP2 Protein is a member of the secreted frizzled-related protein (sFRP) family, indicating its involvement in modulating Wnt signaling pathways. As part of this family, SFRP2 likely interacts with Wnt ligands and frizzled receptors, exerting regulatory effects on crucial cellular processes. The absence of conserved residue(s) required for feature annotation propagation underscores the unique structural characteristics of SFRP2 within the sFRP family. Despite lacking certain conserved elements, SFRP2's specific attributes may contribute to its distinct role in modulating Wnt signaling, potentially influencing cellular activities such as proliferation, differentiation, or tissue development.

Verified Bioactivity

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×103 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    Q80ZA3/NP_808788.1 (Q20-T418)

  • Gene ID
    287526  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin F1 (Q20-T418)
      Accession # Q80ZA3/NP_808788.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINF1; Testis Tissue Sperm-Binding Protein Li 70n; Prev. PEDF; Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1; EPC-1; Proliferation-Inducing Protein 35; Pigment Epithelium-Derived Factor; Serpin F

  • AA Sequence

    QNVPDSSQDSPAPDSTGEPVVEEDDPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGNILLSPLSVATALSALSLGAEQRTESVIHRALYYDLINNPDIHSTYKELLASVTAPEKNFKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRIDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWATKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFVHDIDRELKTIQAVLTVPKLKLSYEGDVTNSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGTSSNPDLQPVRLTFPLDYHLNRPFIFVLRDTDTGALLFIGRILDPSST

  • Molecular Weight

    Approximately 46 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×103 units/mg.

  • Serpin F1 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin F1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Serpin F1 Protein, Rat (HEK293, His)
  • Serpin F1 Protein, Rat (HEK293, His) Product Image