Serpin F1 Protein, Rat (HEK293, His)

Serpin F1 protein is part of the secreted Frizzled-related protein (sFRP) family and regulates the Wnt signaling pathway. As a member, Serpin F1 may interact with Wnt ligands and Frizzled receptors to regulate key cellular processes. Serpin F1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

SFRP2 Protein is a member of the secreted frizzled-related protein (sFRP) family, indicating its involvement in modulating Wnt signaling pathways. As part of this family, SFRP2 likely interacts with Wnt ligands and frizzled receptors, exerting regulatory effects on crucial cellular processes. The absence of conserved residue(s) required for feature annotation propagation underscores the unique structural characteristics of SFRP2 within the sFRP family. Despite lacking certain conserved elements, SFRP2's specific attributes may contribute to its distinct role in modulating Wnt signaling, potentially influencing cellular activities such as proliferation, differentiation, or tissue development.

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

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  Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

Rat

HEK293

C-His

Q80ZA3/NP_808788.1 (Q20-T418)

287526  [NCBI]

Full Length of Mature Protein

QNVPDSSQDSPAPDSTGEPVVEEDDPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGNILLSPLSVATALSALSLGAEQRTESVIHRALYYDLINNPDIHSTYKELLASVTAPEKNFKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRIDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWATKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFVHDIDRELKTIQAVLTVPKLKLSYEGDVTNSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGTSSNPDLQPVRLTFPLDYHLNRPFIFVLRDTDTGALLFIGRILDPSST

Approximately 45.9 kDa

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/nh-12-cell.html