Serpin A8/Angiotensinogen Protein, Mouse (HEK293, His)

Serpin A8 (or angiotensinogen) is an important regulator in the renin-angiotensin system, fine-tuning blood pressure and electrolyte balance. This multifunctional protein acts as a potent vasoconstrictor, affecting vascular smooth muscle and affecting cardiac function via the sympathetic nervous system. Serpin A8/Angiotensinogen Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag.

Serpin A8, commonly known as angiotensinogen, stands as an indispensable component within the intricate framework of the renin-angiotensin system (RAS), playing a pivotal role in the precise regulation of blood pressure, body fluid, and electrolyte homeostasis. This multifaceted protein exerts its influence across various physiological realms, directly impacting vascular smooth muscle as a potent vasoconstrictor. Its reach extends to the cardiovascular system, where it influences cardiac contractility and heart rate through interactions with the sympathetic nervous system. Moreover, angiotensinogen orchestrates dynamic changes in renal function by modulating sodium and water absorption, accomplished by stimulating zona glomerulosa cells in the adrenal cortex to synthesize and release aldosterone. These diverse actions are executed through its adept binding to angiotensin receptors AGTR1 and AGTR2. Beyond its central role in the RAS, angiotensinogen engages in additional interactions, exemplified by its binding to the DEAR/FBXW7-AS1 receptor, further expanding its involvement in intricate cellular signaling networks.

Measured in a cell proliferation assay using HT-29 human coloncancer cells. The ED ₅₀ for this effect is 41.71 ng/mL, corresponding to a specific activity is 2.398×10⁴ Unit/mg.

Mouse

HEK293

C-His

P11859 (D25-V477)

11606  [NCBI]

Full Length of Mature Protein

DRVYIHPFHLLYHNKSTCAQLENPSVETLPESTFEPVPIQAKTSPVNEKTLHDQLVLAAEKLEDEDRKRAAQVAMIANFVGFRMYKMLNEAGSGASGAILSPPALFGTLVSFYLGSLDPTASQLQTLLDVPVKEGDCTSRLDGHKVLAALRAVQGLLVTQGGSSSQTPLLQSIMVGLFTAPGFRLKHSFVQSLALFTPALFPRSLDLSTDPVLATEKINRFIKAVTGWKMNLPLEGVSTDSTLLFNTYVHFQGTMRGFSQLPGVHEFWVDNSISVSVPMISGTGNFQHWSDAQNNFSVTCVPLGERATLLLIQPHCTSDLDRVEALIFRNDLLTWIENPPPRAIRLTLPQLEIRGSYNLQDLLAEDKLPTLLGAEANLSNIGDTNPRVGEVLNSILLELKAGEEEQPTTSVQQPGSPEALDVTLSSPFLFAIYEQDSGTLHFLGRVNNPQSVV

Approximately 50-70 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.