SHMT2 Protein, Human (His)
Based on 1 Customer Validation
The SHMT2 protein catalyzes the cleavage of serine to produce 5,10-methylenetetrahydrofolate for purine synthesis. It binds to mitochondrial DNA and participates in target protein deubiquitination via the BRISC complex. SHMT2 Protein, Human (His) is the recombinant human-derived SHMT2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The SHMT2 protein catalyzes the cleavage of serine to produce 5,10-methylenetetrahydrofolate for purine synthesis. It binds to mitochondrial DNA and participates in target protein deubiquitination via the BRISC complex. SHMT2 Protein, Human (His) is the recombinant human-derived SHMT2 protein, expressed by E. coli , with N-6*His labeled tag.
SHMT2, a versatile enzyme, serves a crucial role in cellular metabolism by catalyzing the cleavage of serine to glycine while concurrently producing 5,10-methylenetetrahydrofolate, an essential intermediate in purine biosynthesis. This process is pivotal for generating one-carbon units, with serine acting as the major contributor to folate-associated one-carbon metabolism. SHMT2's involvement extends to mitochondrial thymidylate biosynthesis, where it aids in preventing uracil accumulation in mtDNA. Additionally, it plays a crucial role in mitochondrial translation by producing 5,10-methylenetetrahydrofolate, a methyl donor essential for the modification of mitochondrial tRNAs. Beyond its mitochondrial functions, SHMT2 participates in the deubiquitination of target proteins as a component of the BRISC complex, demonstrating its multifaceted contributions to cellular processes.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P34897 (S29-H504)
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Gene ID6472
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Molecular Construction
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N-term
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6*His
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SHMT2 (S29-H504)
Accession # P34897 -
C-term
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Protein Length
Partial
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Synonyms
SHMT2; Epididymis Secretory Sperm Binding Protein Li 51e; Prev. SHMT; Serine Hydroxymethyltransferase; Glycine Hydroxymethyltransferase; Serine Hydroxymethylase; Serine Methylase; Threonine Aldolase; MSHMT; Serine Aldolase; Serine Hydroxymethyltransferase
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AA Sequence
SNAAQTQTGEANRGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAVYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPKTGLIDYNQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH
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Molecular Weight
Approximately 54 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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