SHP-1 Protein, Mouse (sf9, His-GST)
SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SHP-1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SHP-1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
SHP-1 protein acts as a critical modulator of signaling pathways initiated by tyrosine phosphorylated cell surface receptors, including KIT and the EGF receptor/EGFR. Additionally, it enhances the inhibition of mast cell activation mediated by the Lilrb4a receptor. The SH2 regions of SHP-1 may engage with other cellular components, influencing its phosphatase activity against interacting substrates. Notably, in collaboration with MTUS1, SHP-1 induces UBE2V2 expression in response to angiotensin II stimulation. Beyond these regulatory functions, SHP-1 plays a key role in hematopoiesis, further emphasizing its significance in the intricate network of cellular processes and signaling events.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
P29351-2 (A207-K597)
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Molecular Construction
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N-term
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His-GST
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SHP-1 (A207-K597)
Accession # P29351-2 -
C-term
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Protein Length
Partial
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Synonyms
PTPN6; Protein-Tyrosine Phosphatase SHP-1; Protein Tyrosine Phosphatase Non-Receptor Type 6; Protein-Tyrosine Phosphatase 1C; PTP-1C; SH-PTP1; HCP; Tyrosine-Protein Phosphatase Non-Receptor Type; SHP-1; Hematopoietic Cell Phosphatase; HCPH; Protein-Tyrosi
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AA Sequence
AFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK
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Predicted Molecular Mass
72.7 kDa
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Molecular Weight
Approximately 63 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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