Siglec-5 Protein, Cynomolgus (HEK293, His)
Siglec-5 Protein is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Siglec-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-5 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Siglec-5 Protein is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Siglec-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-5 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Overexpression of Siglec-5 inhibits the activity of T cell receptor (TCR)-induced transcription factors. In addition, binding of Siglec-5 to the GBS β-protein reduces primary T cell activation and leads to decreased production of cytokines and surface antigens. A soluble form of Siglec-5 enhances cytokine production and cytotoxic granule release by cancer antigen-specific T cells. Siglec-5 is an inhibitory receptor with the potential to mediate SHP-1 and/or SHP-2-dependent signaling in the absence of tyrosine phosphorylation. Plasma Siglec-5 can be used as a diagnostic marker for critical Limb Ischemia and fulminant myocarditis[1][2][3][4].
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-6*His
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Accession
A0A0B4J1D1 (E17-G435)
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Gene ID/
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Molecular Construction
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N-term
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Siglec-5 (E17-G435)
Accession # A0A0B4J1D1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
SIGLEC5; CD33 Antigen-Like 2; Prev. CD33L2; Sialic Acid-Binding Immunoglobulin-Like Lectin 5; OB-BP2; Sialic Acid Binding Ig-Like Lectin 5; SIGLEC-5; CD170 Antigen; CD170; HSALNG0127242; Sialic Acid-Binding Ig-Like Lectin 5; Lnc-SIGLEC5-1; Obesity-Binding
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AA Sequence
EEPGYELQVQKSVTVQEGLCVLVPCSFSYPWNSWYSPSPLYVYWFRDGESQYYAEAVATNNPDRRVKPETQGRFRLLGDVRNKNCSLSIGDARMGDTGNYYFRVERGRNVKYNYVQNKLNLEVTALTEKPDVRFLEPLESGRLTRLSCSLPGSCEVGRPLTFSWTGDVLSPLDPETTGSSELTLTPRPEDHGTNLTCHVKHQGAQVTTERTVQLNVSYAPQNITIFRNGTALEILQNTSTLLVLEGEALRLLCEAPSNPPAHLSWFQASSAPNTTPIANTGILELPRVEFTKEGVFTCRAQHPLGSLHIFLNLSVYSLPQLLGPSCSWEAESLHCSCSFRAWPAPSLCWWLGEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLTSVLKVSCKGWNTYGSQSGSVVLLQGRLNLRTG
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Predicted Molecular Mass
46.8 kDa
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Molecular Weight
Approximately 70-110 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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