2. Recombinant Proteins
  3. CD Antigens Receptor Proteins
  4. Dendritic Cell CD Proteins Siglec
  5. Siglec-5/CD170 Siglec-5
  6. Siglec-5 Protein, Cynomolgus (HEK293, His)

Siglec-5 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P70960
Handling Instructions

Siglec-5 Protein is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Siglec-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-5 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-5 Protein, Cynomolgus (HEK293, His) is 419 a.a., with molecular weight of ~90.0 kDa.

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Description

Siglec-5 Protein is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Siglec-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-5 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-5 Protein, Cynomolgus (HEK293, His) is 419 a.a., with molecular weight of ~90.0 kDa.
Background

Sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Overexpression of Siglec-5 inhibits the activity of T cell receptor (TCR)-induced transcription factors. In addition, binding of Siglec-5 to the GBS β-protein reduces primary T cell activation and leads to decreased production of cytokines and surface antigens. A soluble form of Siglec-5 enhances cytokine production and cytotoxic granule release by cancer antigen-specific T cells. Siglec-5 is an inhibitory receptor with the potential to mediate SHP-1 and/or SHP-2-dependent signaling in the absence of tyrosine phosphorylation. Plasma Siglec-5 can be used as a diagnostic marker for critical Limb Ischemia and fulminant myocarditis[1][2][3][4].
Species

Cynomolgus
Source

HEK293
Tag

C-6*His
Accession

A0A0B4J1D1 (E17-G435)
Gene ID

/
Molecular Construction
N-term
Siglec-5 (E17-G435)
Accession # A0A0B4J1D1
6*His
C-term
Synonyms
Sialic acid-binding Ig-like lectin 5; Siglec-5
AA Sequence

EEPGYELQVQKSVTVQEGLCVLVPCSFSYPWNSWYSPSPLYVYWFRDGESQYYAEAVATNNPDRRVKPETQGRFRLLGDVRNKNCSLSIGDARMGDTGNYYFRVERGRNVKYNYVQNKLNLEVTALTEKPDVRFLEPLESGRLTRLSCSLPGSCEVGRPLTFSWTGDVLSPLDPETTGSSELTLTPRPEDHGTNLTCHVKHQGAQVTTERTVQLNVSYAPQNITIFRNGTALEILQNTSTLLVLEGEALRLLCEAPSNPPAHLSWFQASSAPNTTPIANTGILELPRVEFTKEGVFTCRAQHPLGSLHIFLNLSVYSLPQLLGPSCSWEAESLHCSCSFRAWPAPSLCWWLGEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLTSVLKVSCKGWNTYGSQSGSVVLLQGRLNLRTG

Molecular Weight

Approximately 90.0 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Siglec-5 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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