1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a Protein, Human (G75A, HEK293, His)

SIRP alpha/CD172a Protein, Human (G75A, HEK293, His)

Cat. No.: HY-P74546
Handling Instructions

SIRP alpha V4/CD172a Protein, an immunoglobulin-like cell surface receptor for CD47, acts as a docking protein, facilitating the translocation of PTPN6, PTPN11, and other partners to the plasma membrane. It supports adhesion of cerebellar neurons, promotes neurite outgrowth, and facilitates glial cell attachment. Engaging in negative regulation, it modulates receptor tyrosine kinase-coupled responses, phagocytosis, mast cell activation, and dendritic cell activation. CD47 binding prevents dendritic cell maturation, inhibits cytokine production, and limits new world arenavirus infection. SIRP alpha V4/CD172a also participates in ROS signaling, interacting with THBS1 and various proteins. SIRP alpha/CD172a Protein, Human (G75A, HEK293, His) is the recombinant human-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-His labeled tag and G75A mutation. The total length of SIRP alpha/CD172a Protein, Human (G75A, HEK293, His) is 340 a.a., with molecular weight of 45-55 kDa.

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Description

SIRP alpha V4/CD172a Protein, an immunoglobulin-like cell surface receptor for CD47, acts as a docking protein, facilitating the translocation of PTPN6, PTPN11, and other partners to the plasma membrane. It supports adhesion of cerebellar neurons, promotes neurite outgrowth, and facilitates glial cell attachment. Engaging in negative regulation, it modulates receptor tyrosine kinase-coupled responses, phagocytosis, mast cell activation, and dendritic cell activation. CD47 binding prevents dendritic cell maturation, inhibits cytokine production, and limits new world arenavirus infection. SIRP alpha V4/CD172a also participates in ROS signaling, interacting with THBS1 and various proteins. SIRP alpha/CD172a Protein, Human (G75A, HEK293, His) is the recombinant human-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-His labeled tag and G75A mutation. The total length of SIRP alpha/CD172a Protein, Human (G75A, HEK293, His) is 340 a.a., with molecular weight of 45-55 kDa.

Background

SIRP alpha V4/CD172a Protein, an immunoglobulin-like cell surface receptor for CD47, acts as a docking protein that facilitates the translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. This receptor contributes to diverse cellular processes, including supporting the adhesion of cerebellar neurons, promoting neurite outgrowth, and facilitating glial cell attachment. With a potential role in intracellular signaling during synaptogenesis and synaptic function, SIRP alpha V4/CD172a also engages in negative regulation of receptor tyrosine kinase-coupled responses triggered by cell adhesion, growth factors, or insulin. Furthermore, it mediates the negative modulation of phagocytosis, mast cell activation, and dendritic cell activation. Notably, CD47 binding prevents the maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRP alpha V4/CD172a plays a significant role in antiviral immunity, limiting new world arenavirus infection by decreasing virus internalization. As a receptor for THBS1, it participates in ROS signaling in non-phagocytic cells upon interaction with THBS1, stimulating NADPH oxidase-derived ROS production. The receptor engages in various protein interactions, including binding to PTPN11, GRB2, FGR, JAK2, SCAP1, SCAP2, FYB1, PTK2B, and TRIM2.

Species

Human

Source

HEK293

Tag

C-His

Accession

P78324 (E31-R370, G75A)

Gene ID
Synonyms
Signal-regulatory protein alpha; CD172a; SIRP alpha; SIRPA; MFR; SHPS1; SIRP
AA Sequence

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Molecular Weight

45-55 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SIRP alpha/CD172a Protein, Human (G75A, HEK293, His)
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HY-P74546
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