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SIRP beta 1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

SIRP beta 1 protein negatively regulates receptor tyrosine kinase signaling and recruits SYK for myeloid cell activation. It interacts with TYROBP as a homodimer, facilitating SYK recruitment. This intricate molecular interplay is crucial for modulating myeloid cell activation and regulating cellular signaling. SIRP beta 1 occupies a central position in protein interactions that contribute to its functional role. SIRP beta 1 Protein, Human (HEK293, His) is the recombinant human-derived SIRP beta 1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SIRP beta 1 Protein, Human (HEK293, His)
  • Experimental Validation Results for SIRP beta 1 Protein, Human (HEK293, His)
  • SIRP beta 1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

SIRP beta 1 protein negatively regulates receptor tyrosine kinase signaling and recruits SYK for myeloid cell activation. It interacts with TYROBP as a homodimer, facilitating SYK recruitment. This intricate molecular interplay is crucial for modulating myeloid cell activation and regulating cellular signaling. SIRP beta 1 occupies a central position in protein interactions that contribute to its functional role. SIRP beta 1 Protein, Human (HEK293, His) is the recombinant human-derived SIRP beta 1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

SIRP beta 1 protein, an immunoglobulin-like cell surface receptor, plays a vital role in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Additionally, it participates in the recruitment of the tyrosine kinase SYK, triggering the activation of myeloid cells when associated with TYROBP. Existing as a homodimer with disulfide linkages, SIRP beta 1 interacts specifically with TYROBP, leading to the recruitment of SYK. This intricate molecular interplay positions SIRP beta 1 at the nexus of cellular signaling regulation, highlighting its significance in modulating myeloid cell activation and the intricate network of protein interactions that contribute to its functional role.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP beta 1/CD172b at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SP-D. The ED50 for this effect is 6753 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP beta 1/CD172b at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SP-D.The ED50 for this effect is 6753 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    O00241-1 (E30-E362)

  • Gene ID
    10326  [NCBI]
  • Molecular Construction
    • N-term
    • SIRP beta 1 (E30-E362)
      Accession # O00241-1
    • 6*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    SIRPB1; Signal-Regulatory Protein Beta-1 Isoform 3; Signal Regulatory Protein Beta 1; Signal-Regulatory Protein Beta 1; SIRP-BETA-1; Alternative Protein SIRPB1; CD172b; SIRP-Beta-1 Isoform 3; CD172 Antigen-Like Family Member B; CD172b Antigen; Signal-Regu

  • AA Sequence

    EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHE

  • Molecular Weight

    Approximately 45-55 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP beta 1/CD172b at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SP-D.The ED50 for this effect is 6753 ng/mL.

  • SIRP beta 1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for SIRP beta 1 Protein, Human (HEK293, His)
  • Experimental Validation Results for SIRP beta 1 Protein, Human (HEK293, His)
  • SIRP beta 1 Protein, Human (HEK293, His) Product Image