TAFA5/FAM19A5 Protein, Mouse (His)

As a chemokine-like molecule, TAFA5/FAM19A5 protein activates GPCRs such as S1PR2 and FPR2 to regulate cell proliferation and migration. It induces macrophage chemotactic migration through MAPK3/ERK1 and AKT1 pathways and inhibits TNFSF11/RANKL-induced osteoclast formation. TAFA5/FAM19A5 Protein, Mouse (His) is the recombinant mouse-derived TAFA5/FAM19A5 protein, expressed by E. coli , with N-His labeled tag. The total length of TAFA5/FAM19A5 Protein, Mouse (His) is 89 a.a., with molecular weight of 13-14 kDa.

TAFA5/FAM19A5, functioning as a chemokine-like protein, orchestrates cell proliferation and migration by activating G protein-coupled receptors (GPCRs), notably S1PR2 and FPR2. It stimulates the chemotactic migration of macrophages through the MAPK3/ERK1 and AKT1 pathway while concurrently inhibiting TNFSF11/RANKL-induced osteoclast formation by impeding the up-regulation of osteoclast fusogenic and differentiation genes, a process mediated by the GPCR FPR2. Acting as an adipokine, TAFA5/FAM19A5 negatively regulates vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling. This multifaceted role extends to inhibiting injury-induced cell proliferation and neointima formation in the femoral arteries, underscoring its regulatory impact on diverse cellular processes.

Mouse

E. coli

N-His

Q91WE9-1 (T44-S132)

/

13-14 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Solution

Supplied as a 0.22μm filtered solution of 20mM Tris, 300mM NaCl, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.