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TAOK3 Protein, Human (Active, sf9, His)

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Based on 1 Customer Validation

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for TAOK3 Protein, Human (Active, sf9, His)
  • TAOK3 Protein, Human (Active, sf9, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

TAOK3, a serine/threonine-protein kinase, functions as a regulator of the p38/MAPK14 stress-activated MAPK cascade and the MAPK8/JNK cascade. It acts as an activator of the p38/MAPK14 cascade, particularly in response to DNA damage, where it plays a role in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 cascade. This activation is likely mediated through the phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Moreover, TAOK3 serves as an inhibitor of the basal activity of the MAPK8/JNK cascade and reduces its activation in response to epidermal growth factor (EGF). These regulatory functions highlight TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling.

Verified Bioactivity

Measured by its ability to catalyze 250 μg/mL PKCtide peptide substrate adenosine and 25 μM ATP that incubate at room temperature for 40 minutes. The specific activity is 32.49 nmo/min/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-10*His
  • Accession

    Q9H2K8 (M1-D411)

  • Gene ID
    51347  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His
    • TAOK3 (M1-D411)
      Accession # Q9H2K8
    • C-term
  • Protein Length

    Partial

  • Synonyms

    TAOK3; CTCL-Associated Antigen HD-CL-09; TAO Kinase 3; Kinase From Chicken Homolog A; MAP3K18; Jun Kinase-Inhibitory Kinase; JIK; JNK/SAPK-Inhibitory Kinase; DPK; HKFC-A; Thousand And One Amino Acid Protein 3; Non-Specific Serine/Threonine Protein Kinase;

  • AA Sequence

    MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGD

  • Predicted Molecular Mass

    46.5 kDa

  • Molecular Weight

    Approximately 85 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • TAOK3 Protein, Human (Active, sf9, His) Product Image
    1/16
  • Experimental Validation Results for TAOK3 Protein, Human (Active, sf9, His)
  • TAOK3 Protein, Human (Active, sf9, His) Product Image