TAOK3 Protein, Human (Active, sf9, His)

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

TAOK3, a serine/threonine-protein kinase, functions as a regulator of the p38/MAPK14 stress-activated MAPK cascade and the MAPK8/JNK cascade. It acts as an activator of the p38/MAPK14 cascade, particularly in response to DNA damage, where it plays a role in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 cascade. This activation is likely mediated through the phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Moreover, TAOK3 serves as an inhibitor of the basal activity of the MAPK8/JNK cascade and reduces its activation in response to epidermal growth factor (EGF). These regulatory functions highlight TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling.

Measured by its ability to catalyze 250 μg/mL PKCtide peptide substrate adenosine and 25 μM ATP that incubate at room temperature for 40 minutes. The specific activity is 32.49 nmo/min/mg.

Human

Sf9 insect cells

N-10*His

Q9H2K8 (M1-D411)

51347  [NCBI]

Partial

MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGD

Approximately 54 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.