TetR Protein, E.coli (His)
Based on 1 Customer Validation
TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His) is the recombinant E.coli-derived TetR, expressed by E. coli, with N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His) is the recombinant E.coli-derived TetR, expressed by E. coli, with N-6*His labeled tag.
The TetR protein operates as a repressor for the tetracycline resistance element, with its N-terminal region adopting a helix-turn-helix structure that facilitates DNA binding. Tetracycline binding to TetR induces a conformational change, diminishing the repressor's affinity for the operator sites of the tetracycline resistance gene (tetA) promoter. This dynamic interaction between TetR and tetracycline serves as a regulatory mechanism, modulating the expression of genes associated with tetracycline resistance. In the presence of tetracycline, TetR undergoes alterations in its binding properties, allowing for the derepression of the tetA promoter and enabling the expression of genes involved in resistance to tetracycline.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-6*His
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Accession
P04483 (M1-S207)
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Gene ID56882117
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Protein Length
Full Length
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Synonyms
Tetracycline repressor protein class B from transposon Tn10; tetR
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AA Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
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Predicted Molecular Mass
24.2 kDa
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Molecular Weight
Approximately 25 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 8.0, 8% trehalose, 8% mannitol and 0.02% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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