TFIIB Protein, Human (GST)
TFIIB protein is a general transcription factor involved in the initiation of RNA polymerase II. TFIIB Protein, Human (GST) is the recombinant human-derived TFIIB protein, expressed by E. coli , with N-GST labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
TFIIB protein is a general transcription factor involved in the initiation of RNA polymerase II. TFIIB Protein, Human (GST) is the recombinant human-derived TFIIB protein, expressed by E. coli , with N-GST labeled tag.
The TFIIB Protein is a key general transcription factor involved in the initiation of RNA polymerase II (Pol II)-mediated transcription. It plays a crucial role in pre-initiation complex (PIC) formation and recruits Pol II to the promoter DNA. Together with the TATA box-bound TBP, TFIIB forms the core initiation complex, acting as a bridge between TBP and the Pol II-TFIIF complex. Notably, it is released from the PIC early during the initiation and elongation transition of transcription, reassociating with TBP in the subsequent transcription cycle. TFIIB associates with chromatin at core promoter-specific regions and binds to two distinct DNA core promoter consensus sequence elements known as IIB-recognition elements (BREs). These BREs, located immediately upstream (BREu) and downstream (BREd) of the TATA box element, are recognized in a TBP-independent manner. Additionally, TFIIB exhibits autoacetyltransferase activity, contributing to activated transcription and modulating transcription start site selection. The multifaceted functions of TFIIB highlight its central role in the intricate process of transcription initiation.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-GST
-
Accession
Q00403 (M1-L316)
-
Molecular Construction
-
N-term
-
GST
-
TFIIB (M1-L316)
Accession # Q00403 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
GTF2B; General Transcription Factor TFIIB; General Transcription Factor IIB; S300-II; TFIIB; TF2B; Transcription Initiation Factor IIB; RNA Polymerase II Transcription Factor IIB
-
AA Sequence
MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL
-
Predicted Molecular Mass
61.6 kDa
-
Molecular Weight
Approximately 62 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
