TGF beta 1/TGFB1 Protein, Canine (HEK293, His)

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and interacts with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS) to regulate TGF-β-1 activation. TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is the recombinant canine-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.

The Transforming Growth Factor Beta-1 (TGFB1) proprotein serves as the precursor for both the Latency-associated peptide (LAP) and the active Transforming Growth Factor Beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. It plays a crucial role in maintaining the TGF-beta-1 chain in a latent state during storage within the extracellular matrix. TGFB1 associates non-covalently with TGF-beta-1 and regulates its activation through interactions with 'milieu molecules,' such as LTBP1, LRRC32/GARP, and LRRC33/NRROS, controlling the activation of TGF-beta-1. Notably, the interaction with LRRC33/NRROS regulates TGF-beta-1 activation in macrophages and microglia, while the interaction with LRRC32/GARP controls activation on the surface of activated regulatory T-cells (Tregs). Additionally, the interaction of TGFB1 with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) induces the distortion of the Latency-associated peptide chain, leading to the subsequent release of active TGF-beta-1.

Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/ml (100μl/well) can bind biotinylated LRRC32. The ED₅₀ for this effect is 0.433 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/mL (100 μL/well) can bind biotinylated LRRC32. The ED₅₀ for this effect is 0.433 μg/mL.

Canine

HEK293

C-6*His

P54831 (L30-S390)

403998  [NCBI]

Full Length of Mature Protein

LSTCKTIDMELVKRKRIEAIRGQILSKLRLSSPPSQGEVPPVPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVENTNKIYEKVKKSPHSIYMLFNTSELREAVPEPVLLSRAELRLLRLKLKAEQHVELYQKYSNDSWRYLSNRLLAPSDTPEWLSFDVTGVVRQWLSHGGEVEGFRLSAHCSCDSKDNTLQVDINGFSSSRRGDLATIHGMNRPFLLLMATPLERAQHLHSSRQRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 53-60 kDa(TGFB1 + LAP), 38-45 kDa(LAP) and 15 kDa(TGFB1), based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.