TGF beta 1/TGFB1 Protein, Canine (HEK293, His)

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and interacts with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS) to regulate TGF-β-1 activation. TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is the recombinant canine-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag. The total length of TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is 361 a.a., with molecular weight of ~43.4 kDa.

The Transforming Growth Factor Beta-1 (TGFB1) proprotein serves as the precursor for both the Latency-associated peptide (LAP) and the active Transforming Growth Factor Beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. It plays a crucial role in maintaining the TGF-beta-1 chain in a latent state during storage within the extracellular matrix. TGFB1 associates non-covalently with TGF-beta-1 and regulates its activation through interactions with 'milieu molecules,' such as LTBP1, LRRC32/GARP, and LRRC33/NRROS, controlling the activation of TGF-beta-1. Notably, the interaction with LRRC33/NRROS regulates TGF-beta-1 activation in macrophages and microglia, while the interaction with LRRC32/GARP controls activation on the surface of activated regulatory T-cells (Tregs). Additionally, the interaction of TGFB1 with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) induces the distortion of the Latency-associated peptide chain, leading to the subsequent release of active TGF-beta-1.

Canine

HEK293

C-His

P54831 (L30-S390)

403998  [NCBI]

Approximately 43.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.