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TGF beta 1/TGFB1 Protein, Canine (HEK293, His)

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Based on 1 Customer Validation

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and interacts with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS) to regulate TGF-β-1 activation. TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is the recombinant canine-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Canine
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TGF beta 1/TGFB1 Protein, Canine (HEK293, His)
  • Experimental Validation Results for TGF beta 1/TGFB1 Protein, Canine (HEK293, His)
  • TGF beta 1/TGFB1 Protein, Canine (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and interacts with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS) to regulate TGF-β-1 activation. TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is the recombinant canine-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Transforming Growth Factor Beta-1 (TGFB1) proprotein serves as the precursor for both the Latency-associated peptide (LAP) and the active Transforming Growth Factor Beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. It plays a crucial role in maintaining the TGF-beta-1 chain in a latent state during storage within the extracellular matrix. TGFB1 associates non-covalently with TGF-beta-1 and regulates its activation through interactions with 'milieu molecules,' such as LTBP1, LRRC32/GARP, and LRRC33/NRROS, controlling the activation of TGF-beta-1. Notably, the interaction with LRRC33/NRROS regulates TGF-beta-1 activation in macrophages and microglia, while the interaction with LRRC32/GARP controls activation on the surface of activated regulatory T-cells (Tregs). Additionally, the interaction of TGFB1 with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) induces the distortion of the Latency-associated peptide chain, leading to the subsequent release of active TGF-beta-1.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/ml (100μl/well) can bind biotinylated LRRC32. The ED50 for this effect is 0.433 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/mL (100 μL/well) can bind biotinylated LRRC32. The ED50 for this effect is 0.433 μg/mL.

Technical Parameters

  • Species Canine
  • Source HEK293
  • Tag C-6*His
  • Accession

    P54831 (L30-S390)

  • Gene ID
    403998  [NCBI]
  • Molecular Construction
    • N-term
    • TGFB1 (L30-S390)
      Accession # P54831
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    TGFB1; Transforming Growth Factor Beta1; Prev. TGFB; Camurati-Engelmann Disease; Prev. DPD1; Latency-Associated Peptide; TGFbeta; TGF-Beta-1; CED; TGF-Beta1; Transforming Growth Factor Beta-1 Proprotein; IBDIMDE; Prepro-Transforming Growth Factor Beta-1;

  • AA Sequence

    LSTCKTIDMELVKRKRIEAIRGQILSKLRLSSPPSQGEVPPVPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVENTNKIYEKVKKSPHSIYMLFNTSELREAVPEPVLLSRAELRLLRLKLKAEQHVELYQKYSNDSWRYLSNRLLAPSDTPEWLSFDVTGVVRQWLSHGGEVEGFRLSAHCSCDSKDNTLQVDINGFSSSRRGDLATIHGMNRPFLLLMATPLERAQHLHSSRQRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

  • Predicted Molecular Mass

    41.2 kDa

  • Molecular Weight

    Approximately 53-60 kDa (TGFB1 + LAP) & 38-45 kDa (LAP) & 15 kDa (TGFB1), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/mL (100 μL/well) can bind biotinylated LRRC32. The ED50 for this effect is 0.433 μg/mL.

  • TGF beta 1/TGFB1 Protein, Canine (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TGF beta 1/TGFB1 Protein, Canine (HEK293, His)
  • Experimental Validation Results for TGF beta 1/TGFB1 Protein, Canine (HEK293, His)
  • TGF beta 1/TGFB1 Protein, Canine (HEK293, His) Product Image