Thymidylate Protein, Human (HEK293, His)
Based on 1 Customer Validation
Thymidylate proteins play a key role in DNA synthesis by catalyzing the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This enzymatic process is critical for the production of dTDP, the direct precursor of the DNA building block dTTP. Thymidylate Protein, Human (HEK293, His) is the recombinant human-derived Thymidylate protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Thymidylate proteins play a key role in DNA synthesis by catalyzing the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This enzymatic process is critical for the production of dTDP, the direct precursor of the DNA building block dTTP. Thymidylate Protein, Human (HEK293, His) is the recombinant human-derived Thymidylate protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Thymidylate kinase, an essential enzyme in nucleotide metabolism, catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This phosphorylation reaction is a crucial step in the biosynthesis of deoxythymidine triphosphate (dTTP), a building block for DNA replication and repair. Thymidylate kinase utilizes ATP as the preferred phosphoryl donor, and the reaction is facilitated in the presence of Mg(2+). The enzyme's activity is integral to the maintenance of balanced nucleotide pools and the precise regulation of DNA synthesis in cells.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P23919 (M1-K212)
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Molecular Construction
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N-term
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Thymidylate (M1-K212)
Accession # P23919 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
DTYMK; Thymidylate Kinase; Deoxythymidylate Kinase; Deoxythymidylate Kinase (Thymidylate Kinase); DTMP Kinase; Thymidylate (DTMP) Kinase; CDC8; Tmp_locus_5; TYMK; PP3731; TMPK; CONPM
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AA Sequence
MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK
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Predicted Molecular Mass
24.9 kDa
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Molecular Weight
Approximately 25-31 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM PB, 5% sucrose, 0.05% Tween 80, pH 7.4.
2.Supplied as a 0.22 μm filtered solution of 20mM PB, 150mM NaCl, 10% Sucrose, 0.05% Tween 80, 20% Glycerol, 1mM EDTA, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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