1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TIE-2/CD202b TIE Receptors
  5. TIE-2
  6. TIE-2 Protein, Rat (HEK293, Fc)

TIE-2 Protein, Rat (HEK293, Fc)

Cat. No.: HY-P73438
COA Handling Instructions

TIE-2 protein has growth factor binding activity and is involved in ossification, cAMP response, and estrogen response. It is predicted to be present in a variety of cellular components, as a component of the plasma membrane, and as part of receptor complexes. TIE-2 Protein, Rat (HEK293, Fc) is the recombinant rat-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Rat (HEK293, Fc) is 721 a.a., with molecular weight of 120-130 kDa.

For research use only. We do not sell to patients.

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Description

TIE-2 protein has growth factor binding activity and is involved in ossification, cAMP response, and estrogen response. It is predicted to be present in a variety of cellular components, as a component of the plasma membrane, and as part of receptor complexes. TIE-2 Protein, Rat (HEK293, Fc) is the recombinant rat-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Rat (HEK293, Fc) is 721 a.a., with molecular weight of 120-130 kDa.

Background

TIE-2 Protein possesses growth factor binding activity and is involved in various processes, including endochondral ossification, response to cAMP, and response to estrogen. It is predicted to be located in diverse cellular components such as basolateral plasma membrane, centriolar satellite, and microvillus, and is expected to be an integral component of the plasma membrane and part of a receptor complex. The predicted colocalization with actin filament and stress fiber suggests its potential involvement in cytoskeletal dynamics. Notably, TIE-2 serves as a biomarker for several diseases, including acute kidney tubular necrosis, brain ischemia, gastric ulcer, goiter, and hepatocellular carcinoma. The human ortholog(s) of this gene have been implicated in arteriovenous malformation and multiple cutaneous and mucosal venous malformations. The expression of TIE-2 is biased, with prominent levels observed in lung (RPKM 772.5), heart (RPKM 237.7), and various other tissues.

Biological Activity

Immobilized Rat Tie-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Angiopoietin-2. The ED50 for this effect is 22.89 ng/mL.

Species

Rat

Source

HEK293

Tag

C-hFc

Accession

NP_001099207.1 (A23-L743)

Gene ID

89804  [NCBI]

Molecular Construction
N-term
TIE-2 (A23-L743)
Accession # NP_001099207.1
hFc
C-term
Synonyms
Angiopoietin-1 receptor; CD202b; hTIE2; p140 TEK; Tie2; VMCM
AA Sequence

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKML

Molecular Weight

Approximately 115-130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TIE-2 Protein, Rat (HEK293, Fc)
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