TIM-3/HAVCR2 Protein, Human (HEK293, His)

T cell immunoglobulin mucin-3 is an inhibitory receptor, and inhibits T cell functions. TIM-3 regulates immune responses in autoimmunity and cancer. TIM-3 has multiple different ligands: galectin 9, phosphatidylserine (PtdSer), CEACAM1 and HMGB1. The TIM-3-ligand axis is critical in the pathogenesis of numerous conditions, including autoimmune diseases, infections, cancers, transplant rejection, and chronic inflammation^{[1][2][3][4][5]}. TIM-3/HAVCR2 Protein, Human (HEK293, His) is the recombinant human-derived TIM-3/HAVCR2 protein, expressed by HEK293 , with C-6*His labeled tag.

T cell immunoglobulin mucin-3 (TIM-3) belongs to the Ig superfamily. TIM-3 is usually expressed by multiple murine and human immune cell types. TIM-3 was first discovered on IFN-γ producing Th1 and Tc1 cells. TIM-3 acts as an inhibitory receptor, and inhibits T cell functions. TIM-3 is associated with the regulation of immune responses in autoimmunity and cancer^[1][5].
TIM-3 has multiple different ligands: galectin 9, phosphatidylserine (PtdSer), CEACAM1 and HMGB1, and these ligands bind to different regions on the TIM3 extracellular immunoglobulin V domain. The TIM-3-ligand axis is critical in the pathogenesis of numerous conditions, including autoimmune diseases, infections, cancers, transplant rejection, and chronic inflammation. For example, the binding of TIM-3 with galectin-9 can downregulate Th1 responses^[2][3][4]. Inaddition, dysregulation of Tim-3 expression is associated with autoimmune diseases^[5].

1.Measured by its binding ability in a functional ELISA. Immobilized Human Galectin 9-His at 10 μg/mL (100 μl/well) can bind Human TIM-3-His*: Biotinylated by NHS-biotin prior to testing and the EC₅₀for the effect is 10.5 μg/mL.
2.Immobilized Anti-Human TIM-3 mAb at 2 μg/mL (100 μl/well) can bind TIM-3/HAVCR2, Human (HEK293, His). The ED₅₀ of TIM-3/HAVCR2, Human (HEK293, His) is 5-25 ng/mL.

Human

HEK293

C-6*His

AAL65157.1 (S22-R200)

84868  [NCBI]

Partial

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTLQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

Approximately 40 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.