Trigger factor Protein, E.coli (P.pastoris, His)

Trigger protein is essential for protein export, acting as chaperone and peptidyl prolyl cis-trans isomerase. In its chaperone role, it keeps nascent polypeptides in an open conformation, facilitating correct protein folding. Trigger factor Protein, E.coli (P.pastoris, His) is the recombinant E. coli-derived Trigger factor protein, expressed by P. pastoris , with N-His labeled tag. The total length of Trigger factor Protein, E.coli (P.pastoris, His) is 432 a.a., with molecular weight (affected by relative charge) of ~60 KDa.

Trigger factor protein is integral to protein export, serving a dual role as a chaperone that aids in the proper folding of newly synthesized proteins and as a peptidyl-prolyl cis-trans isomerase. In its chaperone capacity, trigger factor plays a crucial role in maintaining the nascent polypeptide in an open conformation, facilitating the intricate process of protein folding. Simultaneously, its peptidyl-prolyl cis-trans isomerase activity contributes to the isomerization of prolyl peptide bonds, further refining the conformational landscape of the emerging protein. This multifaceted involvement underscores trigger factor's significance in the intricate orchestration of cellular protein homeostasis and export mechanisms.

E.coli

P. pastoris

N-His

B7UJQ9 (M1-432A)

58463319  [NCBI]

MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA

Approximately 60 kDa.The reducing (R) protein migrat es as 60 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.