TrkA Protein, Rat (HEK293, Fc)

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, Fc) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TrkA Protein, Rat (HEK293, Fc) is 418 a.a., with molecular weight of 92-102 kDa.

The TrkA protein, a receptor tyrosine kinase, plays a vital role in the development and maturation of the central and peripheral nervous systems by regulating the proliferation, differentiation, and survival of sympathetic and sensory neurons. Serving as a high-affinity receptor for NGF, its primary ligand, TrkA can also be activated by NTF3/neurotrophin-3, though NTF3 specifically supports axonal extension through NTRK1 without influencing neuron survival. Upon dimeric NGF ligand-binding, TrkA undergoes homodimerization, autophosphorylation, and activation, subsequently recruiting, phosphorylating, and/or activating downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct yet overlapping signaling cascades, steering cell survival and differentiation. Through SHC1 and FRS2, TrkA activates a GRB2-Ras-MAPK cascade controlling cell differentiation and survival, while through PLCG1, it modulates NF-Kappa-B activation and the transcription of genes crucial for cell survival. Additionally, through SHC1 and SH2B1, TrkA controls a Ras-PI3 kinase-AKT1 signaling cascade, further contributing to the regulation of cell survival. In the absence of ligand and activation, TrkA may promote cell death, underscoring the dependence of neuron survival on trophic factors.

Rat

HEK293

C-hFc

P35739 (M1-P418)

59109  [NCBI]

92-102 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.