TrkB Protein, Rat (HEK293, His)

TrkB is a key receptor tyrosine kinase that coordinates multiple neuronal processes in the central and peripheral nervous systems. As a receptor for BDNF and NTF4, it regulates survival through NTF3. TrkB Protein, Rat (HEK293, His) is the recombinant rat-derived TrkB protein, expressed by HEK293 , with C-His labeled tag. The total length of TrkB Protein, Rat (HEK293, His) is 398 a.a., with molecular weight of ~67 kDa.

TrkB, a receptor tyrosine kinase, intricately orchestrates the development and maturation of both the central and peripheral nervous systems, exerting regulatory control over various neuronal processes, including survival, proliferation, migration, differentiation, and synapse formation. Functioning as a receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4, it can alternatively bind NTF3/neurotrophin-3, modulating neuron survival through NTRK2. Upon ligand binding, TrkB undergoes homodimerization, autophosphorylation, and activation, initiating a cascade of downstream events. It recruits, phosphorylates, and activates diverse effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1, which coordinate distinct signaling pathways, including the GRB2-Ras-MAPK cascade for neuronal differentiation, the Ras-PI3 kinase-AKT1 pathway for growth and survival, and PLCG1-regulated pathways for synaptic plasticity. TrkB's involvement in learning and memory encompasses the regulation of short-term synaptic function and long-term potentiation. Additionally, through PLCG1, it activates NF-Kappa-B and transcription of survival-related genes, enabling the suppression of anoikis—a form of apoptosis resulting from the loss of cell-matrix interactions. Notably, TrkB may also contribute to neurotrophin-dependent calcium signaling in glial cells.

Measured by its binding ability in a functional ELISA. Immobilized rat NTRK2-His at 10 μg/mL (100 μL/well) can bind mouse BDNF and the EC₅₀ is 12.2-28.6 ng/mL.

Rat

HEK293

C-His

Q63604-2 (C32-H429)

25054  [NCBI]

Approximately 67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.