TsaE Protein, E.coli
The TsaE protein is essential for the generation of the threonylcarbamoyl group at adenosine position 37 (t(6)A37) in the tRNA that decodes the adenine start codon. It may facilitate the transfer of the threoncarbamoyl moiety from threoncarbamoyl-AMP (TC-AMP) to the N6 group of A37, acting synergistically with TsaD and TsaB. TsaE Protein, E.coli is the recombinant E. coli-derived TsaE protein, expressed by E. coli , with tag free.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The TsaE protein is essential for the generation of the threonylcarbamoyl group at adenosine position 37 (t(6)A37) in the tRNA that decodes the adenine start codon. It may facilitate the transfer of the threoncarbamoyl moiety from threoncarbamoyl-AMP (TC-AMP) to the N6 group of A37, acting synergistically with TsaD and TsaB. TsaE Protein, E.coli is the recombinant E. coli-derived TsaE protein, expressed by E. coli , with tag free.
Background
TsaE stands as a crucial factor in the intricate process of tRNA modification, specifically in the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that decode codons commencing with adenine. Its involvement is proposed in facilitating the transfer of the threonylcarbamoyl moiety from threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, working collaboratively with TsaD and TsaB. While TsaE's exact role in the t(6)A biosynthesis pathway is still elucidated, it is suggested to exert indirect influence, potentially regulating the core enzymatic function of TsaD. Intriguingly, TsaE showcases ATPase activity in vitro, underscoring its dynamic engagement in molecular processes critical for tRNA modification.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag Tag Free
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Accession
P0AF67 (M1-G153)
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Gene ID60902986
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Molecular Construction
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N-term
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TsaE (M1-G153)
Accession # P0AF67 -
C-term
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Protein Length
Full Length
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Synonyms
tsaE; yjeE; b4168; JW4126; tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t; 6A37 threonylcarbamoyladenosine biosynthesis protein TsaE
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AA Sequence
MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG
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Predicted Molecular Mass
16.9 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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