TsaE Protein, E.coli

The TsaE protein is essential for the generation of the threonylcarbamoyl group at adenosine position 37 (t(6)A37) in the tRNA that decodes the adenine start codon. It may facilitate the transfer of the threoncarbamoyl moiety from threoncarbamoyl-AMP (TC-AMP) to the N6 group of A37, acting synergistically with TsaD and TsaB. TsaE Protein, E.coli is the recombinant E. coli-derived TsaE protein, expressed by E. coli , with tag free. The total length of TsaE Protein, E.coli is 153 a.a., with molecular weight of ~16.9 kDa.

TsaE stands as a crucial factor in the intricate process of tRNA modification, specifically in the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that decode codons commencing with adenine. Its involvement is proposed in facilitating the transfer of the threonylcarbamoyl moiety from threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, working collaboratively with TsaD and TsaB. While TsaE's exact role in the t(6)A biosynthesis pathway is still elucidated, it is suggested to exert indirect influence, potentially regulating the core enzymatic function of TsaD. Intriguingly, TsaE showcases ATPase activity in vitro, underscoring its dynamic engagement in molecular processes critical for tRNA modification.

E.coli

E. coli

Tag Free

P0AF67 (1M-153G)

60902986

MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG

Approximately 16.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.