TSLP Protein, Mouse (HEK293, Fc)

TSLP protein, as a cytokine, plays a key role in immune regulation. It induces monocytes to release T-cell attracting chemokines and significantly promotes the maturation of CD11c(+) dendritic cells. TSLP Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TSLP protein, expressed by HEK293 , with C-hFc labeled tag.

The TSLP (thymic stromal lymphopoietin) protein is a cytokine with diverse immunomodulatory functions. It induces the release of T-cell-attracting chemokines from monocytes and promotes the maturation of CD11c(+) dendritic cells, playing a crucial role in the regulation of immune responses. TSLP is known to have implications in allergic inflammation by directly activating mast cells, contributing to the complex network of allergic responses. TSLP exerts its effects by interacting with a receptor complex composed of CRLF2 and IL7R, and the binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruiting IL7R to form the high-affinity ternary complex. These interactions highlight the intricate molecular mechanisms through which TSLP influences immune cell behavior, emphasizing its role in immune system regulation and allergic responses.

1.Immobilized Mouse TSLP at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody. The ED₅₀ of TSLP protein is 3.789 μg/mL, corresponding to a specific activity is 2.64×10⁵ units/mg.
2.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse IL-7RA. The ED₅₀ for this effect is 1.967 ng/mL. Corresponding to a specific activity is 5.084×10⁵ units/mg.

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  Immobilized Mouse TSLP at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody. The ED₅₀ of TSLP protein is 3.789 μg/mL, corresponding to a specific activity is 2.64×10⁵ units/mg.

Mouse

HEK293

C-hFc

Q9JIE6 (Y20-E140)

53603  [NCBI]

Full Length of Mature Protein

YNFSNCNFTSITKIYCNIIFHDLTGDLKGAKFEQIEDCESKPACLLKIEYYTLNPIPGCPSLPDKTFARRTREALNDHCPGYPETERNDGTQEMAQEVQNICLNQTSQILRLWYSFMQSPE

Approximately 42-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.