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USP5 Protein, Human (sf9, His, solution)

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USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (sf9, His, solution) is the recombinant human-derived USP5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for USP5 Protein, Human (sf9, His, solution)
  • USP5 Protein, Human (sf9, His, solution) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (sf9, His, solution) is the recombinant human-derived USP5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

USP5, a deubiquitinating enzyme, demonstrates a marked preference for cleaving both linear and branched multiubiquitin polymers, with a particular affinity for branched structures. It is actively involved in the disassembly of unanchored 'Lys-48'-linked polyubiquitin chains. While USP5 binds linear and 'Lys-63'-linked polyubiquitin with a comparatively lower affinity, its knockdown results in the accumulation of p53/TP53 and an elevation in p53/TP53 transcriptional activity. This effect is attributed to the ability of the unanchored polyubiquitin accumulating in the absence of USP5 to compete with ubiquitinated p53/TP53, but not with MDM2, for recognition by the proteasome.

Verified Bioactivity

Measured by the hydrolysis of Ub-AMC. Reaction was incubated at 37°C for 15 min. The specific activity is 1540.40 pmol/min/μg.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-10*His
  • Accession

    P45974-2 (M1-S835)

  • Gene ID
    8078
  • Molecular Construction
    • N-term
    • USP5 (M1-S835)
      Accession # P45974-2
    • 10*His
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    USP5; Ubiquitin Specific Peptidase 5; Isopeptidase T; IsoT; Ubiquitin‑Specific‑Processing Protease 5; Ubiquitin Carboxyl‑Terminal Hydrolase 5; Deubiquitinating Enzyme 5; Ubiquitin Thioesterase 5; Ubiquitin‑Specific Protease‑5 (Ubiquitin Isopeptidase T); U

  • AA Sequence

    MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

  • Predicted Molecular Mass

    94.7 kDa

  • Molecular Weight

    Approximately 85-94 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • USP5 Protein, Human (sf9, His, solution) Product Image
    1/16
  • Experimental Validation Results for USP5 Protein, Human (sf9, His, solution)
  • USP5 Protein, Human (sf9, His, solution) Product Image