VCAM-1/CD106 Protein, Human (Biotinylated, HEK293, hFc)

VCAM-1/CD106 proteins expressed on endothelial cells critically regulate leukocyte adhesion through integrin interactions, initiating calcium channel activation and RAC1-mediated transendothelial migration during inflammation. VCAM-1/CD106 Protein, Human (Biotinylated, HEK293, hFc) is the recombinant human-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-hFc labeled tag.

VCAM-1/CD106 is a cell adhesion glycoprotein predominantly expressed on endothelial cells, playing a pivotal role in immune surveillance and inflammation. As a major regulator of leukocyte adhesion to the endothelium, it interacts with various integrins, influencing immune cell interactions. During inflammatory responses, VCAM-1 binds ligands on activated endothelial cells, initiating calcium channel activation and RAC1 small GTPase activation, facilitating leukocyte transendothelial migration. Additionally, VCAM-1 acts as a quality-control checkpoint for entry into the bone marrow, providing a 'don't-eat-me' signal in the context of major histocompatibility complex (MHC) class-I presentation.

Human

HEK293

C-hFc

P19320 (F25-E697)

7412  [NCBI]

Extracellular Domain

Biotin

The biotin is covalently conjugated to the primary amine groups of the protein using chemical labeling methods.

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSP

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.