VEGF-BB Protein, Human (HEK293, Fc)

VEGF-BB protein is an endothelial cell-specific growth factor with VEGF-B167 and VEGF-B186 isoforms and has a unique binding ability to heparin and Neuropilin-1. Structural homodimerization linked by disulfide bonds allows the formation of heterodimers with VEGF, emphasizing its role in regulating endothelial cell function. VEGF-BB Protein, Human (HEK293, Fc) is the recombinant human-derived VEGF-BB protein, expressed by HEK293 , with N-hFc labeled tag. The total length of VEGF-BB Protein, Human (HEK293, Fc) is 186 a.a., with molecular weight of 40 & 60 kDa, respectively.

VEGF-BB Protein serves as a growth factor specifically for endothelial cells. The isoform VEGF-B167 exhibits binding capabilities with heparin and neuropilin-1, while the binding of VEGF-B186 to neuropilin-1 is subject to regulation by proteolysis. Structurally, VEGF-BB exists as a homodimer connected by disulfide bonds and has the capacity to form a heterodimer with VEGF. This molecular configuration underscores its distinct role in regulating endothelial cell functions and highlights the intricacies involved in its binding interactions, contributing to the complexity of vascular processes.

Human

HEK293

N-hFc

P49765 (P22-A207)

7423  [NCBI]

PVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

40&60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.