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VEGF-B Protein, Human (HEK293, Fc)

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

VEGF-B protein is an endothelial cell-specific growth factor with VEGF-B167 and VEGF-B186 isoforms and has a unique binding ability to heparin and Neuropilin-1. VEGF-B Protein, Human (HEK293, Fc) is the recombinant human-derived VEGF-B protein, expressed by HEK293 , with N-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for VEGF-B Protein, Human (HEK293, Fc)
  • VEGF-B Protein, Human (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

VEGF-B protein is an endothelial cell-specific growth factor with VEGF-B167 and VEGF-B186 isoforms and has a unique binding ability to heparin and Neuropilin-1. VEGF-B Protein, Human (HEK293, Fc) is the recombinant human-derived VEGF-B protein, expressed by HEK293 , with N-hFc labeled tag.

Background

VEGF-B, or vascular endothelial growth factor B, is a protein that plays a role in cell survival and possibly in cardiac and metabolic function. VEGF-B, unlike VEGF-A, acts primarily as an angiogenic (blood vessel growth) factor and does not strongly induce blood vessel growth in most tissues. However, VEGF-B can promote coronary blood vessel growth and protect the heart from ischemic injury. VEGF-B also has survival effects on a variety of cell types, including neurons and cardiomyocytes, by inhibiting apoptosis (programmed cell death).

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-hFc
  • Accession

    P49765 (P22-A207)

  • Gene ID
    7423  [NCBI]
  • Molecular Construction
    • N-term
    • hFc
    • VEGF-BB (P22-A207)
      Accession # P49765
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    VEGFB; Vascular Endothelial Growth Factor B; VRF; VEGF-Related Factor; VEGFL; VEGF-B

  • AA Sequence

    PVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

  • Predicted Molecular Mass

    45.7 kDa

  • Molecular Weight

    Approximately 40 kDa & 60 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    VEGF-B Protein, Human (HEK293, Fc) purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 May 20;25(10):5559. [Abstract]

    Customer Validation
     The conditioned media from RT4 (n=7) and T24 (n=11) cells transfected with siPSMB4 were collected. The angiogenesis factors of VEFG-121, VEGF-165, and VEGF-B (2 µg/mL) were added into the condition media. Tube formation was observed after 6 h.

  • VEGF-B Protein, Human (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for VEGF-B Protein, Human (HEK293, Fc)
  • VEGF-B Protein, Human (HEK293, Fc) Product Image

  • VEGF-B Protein, Human (HEK293, Fc) purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 May 20;25(10):5559. [Abstract]

    The conditioned media from RT4 (n=7) and T24 (n=11) cells transfected with siPSMB4 were collected. The angiogenesis factors of VEFG-121, VEGF-165, and VEGF-B (2 µg/mL) were added into the condition media. Tube formation was observed after 6 h.