Vif Protein, HIV-1 group M subtype B (P. pastoris, His)
Vif Protein, HIV-1 group M subtype B (P. pastoris, His) is the recombinant virus-derived Vif protein, expressed by P. pastoris, with N-SUMO & N-6*His tag.
- Species: Virus
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Vif Protein, HIV-1 group M subtype B (P. pastoris, His) is the recombinant virus-derived Vif protein, expressed by P. pastoris, with N-SUMO & N-6*His tag.
Background
Vif counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G by promoting their ubiquitination and degradation. It acts as a substrate recognition component of an E3 ubiquitin-protein ligase complex: Vif hijacks a host cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) and the transcription coactivator CBFB/CBF-beta to form an active E3 ubiquitin-protein ligase complex that targets APOBEC3G and APOBEC3F for polyubiquitination, leading to their proteasomal degradation. Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. Vif may interact directly with APOBEC3G mRNA to inhibit its translation. Association with CBFB/CBF-beta also inhibits the transcription coactivator activity of CBFB/CBF-beta. Vif appears to play a role in viral morphology by affecting the stability of the viral nucleoprotein core. Additionally, Vif contributes to the G2 cell cycle arrest observed in HIV-infected cells.
Technical Parameters
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Species Virus
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Source P. pastoris
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Tag N-SUMO;N-6*His
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Accession
P69721 (M1-H192)
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Gene ID155495
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Molecular Construction
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N-term
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SUMO
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6*His
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(M1-H192)
Accession # P69721 -
C-term
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Protein Length
Full Length
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Synonyms
vif; Virion infectivity factor; Vif; SOR protein; p17; p7
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AA Sequence
MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSGKARGWFYRHHYESPHPRISSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPELADQLIHLYYFDCFSDSAIRKALLGHIVSPRCEYQAGHNKVGSLQYLALAALITPKKIKPPLPSVTKLTEDRWNKPQKTKGHRGSHTMNGH
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Predicted Molecular Mass
38.5 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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