WISP1/CCN4 Protein, Human (HEK293, His)

WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as proliferation, migration, and differentiation. It is implicated in diseases like cancer and fibrosis. The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research. WISP1/CCN4 Protein, Human (HEK293, His) is the recombinant human-derived WISP1/CCN4, expressed by HEK293, with C-His labeled tag.

WISP1/CCN4 is a downstream regulator in the Wnt/Frizzled-signaling pathway, intricately associated with cell survival mechanisms. It plays a crucial role in mitigating p53-mediated apoptosis triggered by DNA damage, achieved through the activation of AKT kinase. Moreover, WISP1/CCN4 exerts its influence on apoptosis by up-regulating the anti-apoptotic Bcl-X(L) protein. Notably, it exhibits an affinity for skin and melanoma fibroblasts, engaging in binding interactions with proteoglycans like decorin and biglycan on skin fibroblasts in in vitro settings. These multifaceted activities underscore the importance of WISP1/CCN4 in cellular processes and its potential implications in the regulation of cell survival pathways.

Human

HEK293

C-His

O95388 (T23-N367)

8840  [NCBI]

Full Length of Mature Protein

TALSPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN

Approximately 55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.