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WISP1/CCN4 Protein, Human (HEK293, His)

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WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as proliferation, migration, and differentiation. It is implicated in diseases like cancer and fibrosis. The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research. WISP1/CCN4 Protein, Human (HEK293, His) is the recombinant human-derived WISP1/CCN4, expressed by HEK293, with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • WISP1/CCN4 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as proliferation, migration, and differentiation. It is implicated in diseases like cancer and fibrosis. The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research. WISP1/CCN4 Protein, Human (HEK293, His) is the recombinant human-derived WISP1/CCN4, expressed by HEK293, with C-His labeled tag.

Background

WISP1/CCN4 is a downstream regulator in the Wnt/Frizzled-signaling pathway, intricately associated with cell survival mechanisms. It plays a crucial role in mitigating p53-mediated apoptosis triggered by DNA damage, achieved through the activation of AKT kinase. Moreover, WISP1/CCN4 exerts its influence on apoptosis by up-regulating the anti-apoptotic Bcl-X(L) protein. Notably, it exhibits an affinity for skin and melanoma fibroblasts, engaging in binding interactions with proteoglycans like decorin and biglycan on skin fibroblasts in in vitro settings. These multifaceted activities underscore the importance of WISP1/CCN4 in cellular processes and its potential implications in the regulation of cell survival pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    O95388 (T23-N367)

  • Gene ID
    8840  [NCBI]
  • Molecular Construction
    • N-term
    • WISP1 (M1-N367)
      Accession # O95388
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCN4; CCN Family Member 4; Prev. WISP1; WNT1 Induced Secreted Protein 1; Prev. WISP1-OT1; WISP1 Overlapping Transcript 1; Prev. WISP1-UT1; Wnt-1-Induced Secreted Protein; WISP-1; WISP1tc; WISP1 Overlapping Transcript 1 (Non-Protein Coding); WISP1c; WNT1 I

  • AA Sequence

    TALSPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN

  • Predicted Molecular Mass

    39 kDa

  • Molecular Weight

    Approximately 55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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