WNK4 Protein, Human (Inactive, sf9, His, GST)
WNK4 Protein, Human (Inactive, sf9, His, GST) is the recombinant human-derived WNK4, expressed by sf9 insect cells , with His, GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
WNK4 Protein, Human (Inactive, sf9, His, GST) is the recombinant human-derived WNK4, expressed by sf9 insect cells , with His, GST labeled tag. ,
Background
WNK4 is a serine/threonine-protein kinase that serves as a key component of the WNK4-SPAK/OSR1 kinase cascade, playing a critical role in ion transport regulation in the distal nephron and blood pressure control (By similarity). Within this cascade, WNK4 phosphorylates and activates downstream kinases OXSR1/OSR1 and STK39/SPAK, which in turn modulate the activity of ion cotransporters such as SLC12A1/NKCC2 and SLC12A3/NCC. Functioning as a molecular switch, WNK4 balances renal salt reabsorption and potassium secretion by regulating transporters and channels including SLC12A3/NCC and KCNJ1/ROMK (By similarity). Independently of its kinase activity, WNK4 acts as a scaffold protein to negatively regulate membrane localization of various transporters/channels (e.g., CFTR, TRPV4) via clathrin-dependent endocytosis (By similarity). It also inhibits epithelial sodium channel (ENaC) activity through a kinase-independent mechanism (By similarity) and may phosphorylate NEDD4L.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag His;GST
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Accession
Q96J92-1 (L2-E444)
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Gene ID65266
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Protein Length
Partial
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Synonyms
WNK4; WNK Lysine Deficient Protein Kinase 4; PRKWNK4; Serine/Threonine-Protein Kinase WNK4; Protein Kinase Lysine-Deficient 4; Protein Kinase With No Lysine 4; Protein Kinase, Lysine Deficient 4; PHA2B
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AA Sequence
LASPATETTVLMSQTEADLALRPPPPLGTAGQPRLGPPPRRARRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSLPASPAPDPPDPPDSAGPGPARSPPPSSKEPPEGTWTEGAPVKAAEDSARPELPDSAVGPGSREPLRVPEAVALERRREQEEKEDMETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGRKPNSFHKVKIPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAE
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Predicted Molecular Mass
77.3 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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