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YKT6 Protein, Human (His)

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Based on 1 Customer Validation

YKT6 is a vesicle soluble NSF attachment protein receptor (v-SNARE) that mediates vesicle docking and fusion in the endoplasmic reticulum to Golgi trafficking, forming a SNARE complex with GOSR1, GOSR2, and STX5. It is also involved in the transport of early/recycling endosomes to the trans-Golgi network, forming another SNARE complex with BET1L, GOSR1 and STX5. YKT6 Protein, Human (His) is the recombinant human-derived YKT6 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for YKT6 Protein, Human (His)
  • YKT6 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

YKT6 is a vesicle soluble NSF attachment protein receptor (v-SNARE) that mediates vesicle docking and fusion in the endoplasmic reticulum to Golgi trafficking, forming a SNARE complex with GOSR1, GOSR2, and STX5. It is also involved in the transport of early/recycling endosomes to the trans-Golgi network, forming another SNARE complex with BET1L, GOSR1 and STX5. YKT6 Protein, Human (His) is the recombinant human-derived YKT6 protein, expressed by E. coli , with N-6*His labeled tag.

Background

YKT6, a vesicular soluble NSF attachment protein receptor (v-SNARE), plays a pivotal role in mediating vesicle docking and fusion with specific acceptor cellular compartments. It functions in endoplasmic reticulum to Golgi transport, contributing to a SNARE complex alongside GOSR1, GOSR2, and STX5. Additionally, YKT6 is involved in early/recycling endosome to trans-Golgi network transport, forming another SNARE complex with BET1L, GOSR1, and STX5. Beyond its role in membrane fusion events, YKT6 exhibits S-palmitoyl transferase activity, adding a layer of complexity to its functional repertoire. Identified in two distinct SNARE complexes, YKT6's involvement underscores its significance in orchestrating vesicular trafficking and membrane fusion processes within the cellular transport network.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    O15498-1 (M1-M198)

  • Gene ID
    10652  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • YKT6 (M1-M198)
      Accession # O15498-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    YKT6; YKT6 Vesicular SNARE Protein; YKT6 V-SNARE Homolog; Synaptobrevin Homolog YKT6; R-SNARE; YKT6 V-SNARE Homolog (S. Cerevisiae); YKT6; S. Cerevisiae; Homolog Of; YKT6 V-SNARE Protein; SNARE Protein Ykt6

  • AA Sequence

    MKLYSLSVLYKGEAKVVLLKAAYDVSSFSFFQRSSVQEFMTFTSQLIVERSSKGTRASVKEQDYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDEFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM

  • Predicted Molecular Mass

    24.5 kDa

  • Molecular Weight

    Approximately 28 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • YKT6 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for YKT6 Protein, Human (His)
  • YKT6 Protein, Human (His) Product Image