2. Stem Cell/Wnt
  3. Hedgehog
  4. RUSKI-201 dihydrochloride

RUSKI-201 dihydrochloride is a potent and specific Hedgehog acyltransferase (Hhat) inhibitor with an IC50 of 0.20 μM. RUSKI-201 dihydrochloride is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 dihydrochloride is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function.

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RUSKI-201 dihydrochloride Chemical Structure

RUSKI-201 dihydrochloride Chemical Structure

CAS No. : 2320262-09-5

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RUSKI-201 dihydrochloride Related Antibodies

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Description

RUSKI-201 dihydrochloride is a potent and specific Hedgehog acyltransferase (Hhat) inhibitor with an IC50 of 0.20 μM. RUSKI-201 dihydrochloride is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 dihydrochloride is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function[1].

In Vitro

RUSKI-201 (0.5-25 μM; 48 hours) has no effect on cell viability at concentrations >25 μM in Shh-Light2 cells derived from NIH3T3 cells stably transfected with a Gli-responsive firefly luciferase HEK-293 cells stably overexpressing Shh[1].
RUSKI-201 inhibits signaling in H520, Panc-1, and MCF-7 coculture with Shh-Light2 cells (IC50=4.8±0.60 μM, 7.8±1.3 μM, and 8.5±0.65 μM, respectively)[1]RUSKI-201 (0.01-10 μM; 24 hours) induces a selective inhibition of Shh palmitoylation and does not affect global palmitoylation levels in HEK-293 Shh+ cells treated with RUSKI-201 followed by YnPal and functionalized with AzTB[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RUSKI-201 dihydrochloride Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK-293 Shh+ cells
Concentration: 0.01 μM, 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM
Incubation Time: 24 hours
Result: Decreased YnPal-Shh expression without α-Shh change;
Capable of blocking Hh signaling from Shh overexpressing cells.
Molecular Weight

430.43

Formula

C20H29Cl2N3OS
CAS No.
SMILES

CCC(CNCC(N1CCC2=C(C1C3=NC(C)=CC=C3)C=CS2)=O)C.Cl.Cl
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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RUSKI-201 dihydrochloride Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RUSKI-2012320262-09-5RUSKI201RUSKI 201HedgehogHhatHh palmitoylationH520Panc-1MCF-7Shh-Light2tumorInhibitorinhibitorinhibit

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