Sakamototide substrate peptide TFA
Based on 1 Customer Validation
Sakamototide substrate peptide TFA is a peptide substrate for members of the AMPK family of kinases, used in kinase activity assays.
For research use only. We do not sell to patients.
- Purity: 98.70%
- Formula: C71H123F3N30O25
- Molecular Weight:1853.92
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
Chemical Information
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Appearance Solid
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Molecular Weight 1853.92
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Formula C71H123F3N30O25
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Color White to off-white
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Sequence
Ala-Leu-Asn-Arg-Thr-Ser-Ser-Asp-Ser-Ala-Leu-His-Arg-Arg-Arg
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Sequence Shortening
ALNRTSSDSALHRRR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 100 mg/mL (53.94 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (26.97 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Banerjee S,et al. Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem J. 2014 Jan 1;457(1):215-25. [Content Brief]
[2]. Banerjee S, et al. Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatasecomplex and the SCFβTrCP E3 ubiquitin ligase. Biochem J. 2014 Jul 15;461(2):233-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5394 mL | 2.6970 mL | 5.3940 mL | 13.4849 mL |
| 5 mM | 0.1079 mL | 0.5394 mL | 1.0788 mL | 2.6970 mL | |
| 10 mM | 0.0539 mL | 0.2697 mL | 0.5394 mL | 1.3485 mL | |
| 15 mM | 0.0360 mL | 0.1798 mL | 0.3596 mL | 0.8990 mL | |
| 20 mM | 0.0270 mL | 0.1348 mL | 0.2697 mL | 0.6742 mL | |
| 25 mM | 0.0216 mL | 0.1079 mL | 0.2158 mL | 0.5394 mL | |
| 30 mM | 0.0180 mL | 0.0899 mL | 0.1798 mL | 0.4495 mL | |
| 40 mM | 0.0135 mL | 0.0674 mL | 0.1348 mL | 0.3371 mL | |
| 50 mM | 0.0108 mL | 0.0539 mL | 0.1079 mL | 0.2697 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.