SCO-NHS carbonate
Based on 1 Customer Validation
SCO-NHS carbonate is a click chemistry agent.
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- Reinheit : 95.0%
- CAS. Nr.: 2925279-71-4
- Formel: C13H15NO5
- Molecular Weight:265.26
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Speicherung:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
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Help & FAQs
Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 2925279-71-4
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Appearance Solid
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Molecular Weight 265.26
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Formel C13H15NO5
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Color White to off-white
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SMILES
O=C(OC1C#CCCCCC1)ON2C(CCC2=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 250 mg/mL (942.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
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Data Sheet (245 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7699 mL | 18.8494 mL | 37.6989 mL | 94.2472 mL |
| 5 mM | 0.7540 mL | 3.7699 mL | 7.5398 mL | 18.8494 mL | |
| 10 mM | 0.3770 mL | 1.8849 mL | 3.7699 mL | 9.4247 mL | |
| 15 mM | 0.2513 mL | 1.2566 mL | 2.5133 mL | 6.2831 mL | |
| 20 mM | 0.1885 mL | 0.9425 mL | 1.8849 mL | 4.7124 mL | |
| 25 mM | 0.1508 mL | 0.7540 mL | 1.5080 mL | 3.7699 mL | |
| 30 mM | 0.1257 mL | 0.6283 mL | 1.2566 mL | 3.1416 mL | |
| 40 mM | 0.0942 mL | 0.4712 mL | 0.9425 mL | 2.3562 mL | |
| 50 mM | 0.0754 mL | 0.3770 mL | 0.7540 mL | 1.8849 mL | |
| 60 mM | 0.0628 mL | 0.3142 mL | 0.6283 mL | 1.5708 mL | |
| 80 mM | 0.0471 mL | 0.2356 mL | 0.4712 mL | 1.1781 mL | |
| 100 mM | 0.0377 mL | 0.1885 mL | 0.3770 mL | 0.9425 mL |
SCO-NHS carbonate Related Classifications
HY-157012 Related Classifications
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