2. Immunology/Inflammation NF-κB MAPK/ERK Pathway Stem Cell/Wnt Apoptosis
  3. Toll-like Receptor (TLR) NF-κB p38 MAPK ERK IKK TNF Receptor Interleukin Related
  4. SMU-14a

SMU-14a is a selective Toll-like receptor 3 (TLR3) inhibitor wirh an IC50 of 0.18 μM. SMU-14a reduces phosphorylation of p65, ERK, and TBK1 via NF-κB, MAPK, and IRF3 signaling pathways. SMU-14a inhibits IL-6 secretion in mouse peritoneal macrophages, downregulates TNF-α in human peripheral blood monocytes and decreases serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. SMU-14a can be used for the research of acute hepatitis.

For research use only. We do not sell to patients.

SMU-14a

SMU-14a Chemical Structure

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SMU-14a Related Antibodies

Top Publications Citing Use of Products

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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ERK ERK1 ERK2 ERK5 ERK8

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IKK-α IKK-β IKK IKKε TBK1

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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Description

SMU-14a is a selective Toll-like receptor 3 (TLR3) inhibitor wirh an IC50 of 0.18 μM. SMU-14a reduces phosphorylation of p65, ERK, and TBK1 via NF-κB, MAPK, and IRF3 signaling pathways. SMU-14a inhibits IL-6 secretion in mouse peritoneal macrophages, downregulates TNF-α in human peripheral blood monocytes and decreases serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. SMU-14a can be used for the research of acute hepatitis[1].

IC50 & Target[1]

TLR3

0.18 μM (IC50)

TBK1

 

IL-6

 

p65

 

In Vitro

SMU-14a (1-10 μM; 24 h) potently inhibits TLR3 activity in HEK-Blue hTLR3 cells induced by Poly I:C (HY-107202) with an IC50 of 0.18 ± 0.02 μM and does not affect TLR2, TLR4, or TLR7 activity[1].
SMU-14a (0.01-1 μM; 24 h) dose-dependently reduces Poly I:C-induced IL-6 secretion in RAW 264.7 cells[1].
SMU-14a (0.01-1 μM; 24 h) dose-dependently reduces Poly I:C-induced TNF-α secretion in human peripheral blood mononuclear cells[1].
SMU-14a (0.01-1 μM; 24 h) does not induce cytotoxicity in human peripheral blood mononuclear cells after 24 h of incubation[1].
SMU-14a (0.01-1 μM; 4 h) dose-dependently inhibits Poly I:C-induced phosphorylation of p65, ERK, and TBK1 through the NF-κB, MAPK, and IRF3 signaling pathways in RAW 264.7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SMU-14a Related Antibodies

Western Blot Analysis[1]

Cell Line: RAW 264.7 cells
Concentration: 0.01, 0.1, 1 μM
Incubation Time: 4 h
Result: Dose-dependently reversed Poly I:C-induced phosphorylation of p65 (NF-κB pathway), ERK (MAPK pathway), and TBK1 (IRF3 pathway) in RAW 264.7 cells.
In Vivo

SMU-14a (50 mg/kg; i.v.; single dose) significantly reduces serum liver enzyme and inflammatory cytokine levels, and attenuates histopathological liver damage, demonstrating potent hepatoprotective effects in a mouse model of Concanavalin A (HY-P2149)-induced acute hepatitis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (male, 6-8 weeks old, 20-22 g, tail vein injection of 20 mg/kg Concanavalin A)[1]
Dosage: 50 mg/kg
Administration: single dose (0.5 hours before Concanavalin A challenge)
Result: Significantly reduced serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the inflammatory cytokine IL-6 compared to the untreated model group.
Reduced hepatic congestion, edema, and inflammatory cell infiltration, with preservation of liver lobule structure relative to the model group.
Molecular Weight

380.44

Formula

C24H20N4O
SMILES

O=C(C1=CC2=C(NC3=C2C(C)=C(C=NC=C4)C4=C3C)C=C1)NC5=CC=CC=C5N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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SMU-14a Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMU-14aToll-like Receptor (TLR)NF-κBp38 MAPKERKIKKTNF ReceptorInterleukin RelatedNuclear factor-κBNuclear factor-kappaBExtracellular signal regulated kinasesIκB kinaseI kappa B kinase Tumor Necrosis Factor ReceptorTNFRILTLR3Toll-like receptor 3human peripheral blood mononuclear cellsTBK1IRF3mouse peritoneal macrophagesp65MAPKInhibitorinhibitorinhibit

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