Subtilisin, bacillus licheniformis
Based on 1 Customer Validation
Subtilisin, bacillus licheniformis (Bacillopeptidaseb) is a serine endopeptidase that cleaves peptide bonds with broad substrate specificity. Subtilisin, bacillus licheniformis exhibits P1 site preference (the first residue at the N-terminus of the cleavage site). Subtilisin, bacillus licheniformis can be used in protein hydrolysis, detergents, proteomics, and other fields.
For research use only. We do not sell to patients.
- CAS No.: 9014-01-1
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-P2729
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Cathepsin B, Bovine spleenCysteine Protease -
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AminopeptidaseEnzyme
Biological Activity
Description
In Vitro
Product Information
This product is a proteolytic enzyme derived from Bacillus licheniformis 2709, which was obtained through mutagenic breeding of bacterial protoplasts. It is produced via deep fermentation, extraction, and refinement processes. The primary enzymatic component is Bacillus licheniformis protease, a serine-type endopeptidase that hydrolyzes peptide chains in protein molecules to produce polypeptides or amino acids. It exhibits strong protein-degrading capability. Optimal reaction conditions: temperature 50-55°C, pH 8.0-10.0.
Instructions
This product is water-soluble and can be dissolved in water or according to specific experimental literature references. It is susceptible to inhibition and damage by heavy metal ions (Fe³⁺, Cu²⁺, Hg⁺, Pb⁺, etc.) and oxidizing agents. Avoid contact with these substances during use and storage.
Subtilisin (Compound proteinase) (30 mg/mL; 2.5-7 d) selectively acylates disaccharides in anhydrous dimethylformamide[1].
Subtilisin (Compound proteinase) (1.8 g; 20-72 h) selectively acylates monosaccharides in anhydrous dimethylformamide[1].
Subtilisin (Compound proteinase) proteinase acylates D-glucose of flavanol isoquercetin (1) in the presence of activated ester trifluoroethyl butyrate (TFEB)[3].
Subtilisin (Compound proteinase) improves the solubility of protein and fatty acids, and restores polypeptides and unsaturated fatty acids[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.4.21.62
Enzyme Activity
≥1000000 U/g
Unit Definition
One unit is defined as the amount of enzyme that will hydrolysis of casein produced 1μg tyrosine per min at 40°C and pH 10.5.
Chemical Information
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CAS No. 9014-01-1
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Subtilisin, bacillus licheniformis]
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Synonyms
Bacillopeptidaseb
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Protocols
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Research Protocol for Omics Data Analysis Pipelines
Omics data analysis pipelines convert raw high-throughput measurements from genomics, transcriptomics, epigenomics, proteomics, metabolomics, or single-cell assays into quality-controlled, statistically tested, biologically interpretable results. A reproducible omics pipeline requires predefined experimental metadata, raw-data quality control, modality-specific preprocessing, normalization, statistical modeling, multiple-testing correction, biological annotation, and independent validation. RNA-seq pipelines commonly include read QC, alignment or pseudoalignment, quantification, normalization, and differential-expression testing, while single-cell pipelines additionally require cell-level QC, normalization, dimensionality reduction, clustering, cell annotation, and sample-aware differential testing. Multi-omics integration can connect molecular layers such as transcriptome, proteome, metabolome, and epigenome, but unresolved problems include batch effects, missing values, unequal featu
Purity & Documentation
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Data Sheet (273 KB)
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SDS (923 KB)
- English - EN (923 KB)
- Français - FR (923 KB)
- Deutsch - DE (923 KB)
- Norwegian - NO (923 KB)
- Español - ES (923 KB)
- Swedish - SV (923 KB)
- Italian - IT (923 KB)
- Korean - KR (923 KB)
- Portuguese - PT (923 KB)
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Handling Instructions (2659 KB)
References
[2]. Philipp M, et al. Inhibition of subtilisin by substituted arylboronic acids. FEBS Lett. 1981 Oct 12;133(1):36-8. [Content Brief]
[4]. Kim MJ, et al. Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice. Int J Mol Sci. 2022 Apr 5;23(7):4015. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Subtilisin, bacillus licheniformis Related Classifications
HY-E70076 Related Classifications
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