Suc-Ala-Ala-Pro-Val-AMC
Based on 1 Customer Validation
Suc-Ala-Ala-Pro-Val-AMC is a fluorescent substrate for elastase. Suc-Ala-Ala-Pro-Val-AMC can be hydrolyzed by these elastases to release the fluorescent substance 7-amino-4-methylcoumarin. Suc-Ala-Ala-Pro-Val-AMC is used for in vitro elastase activity assays and can also be applied in studies related to chronic wounds.
For research use only. We do not sell to patients.
- Purity: 99.08%
- CAS No.: 113277-37-5
- Formula: C30H39N5O9
- Molecular Weight:613.66
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Suc-Ala-Ala-Pro-Val-AMC-immobilized pNC biosensors detect human neutrophil elastase with the highest sensitivity (0.015 U/mL) among the tested cellulosic and nanocellulosic biosensors using the target reagent, followed by pNCC (0.125 U/mL) and pCPC (0.500 U/mL)[2].
Suc-Ala-Ala-Pro-Val-AMC-immobilized pNC biosensors sequester the highest percentage (67%) of human neutrophil elastase from wound-like fluid among the tested cellulosic and nanocellulosic biosensors using the target reagent[2].
Suc-Ala-Ala-Pro-Val-AMC loading is highest (88.13 μg/mg) on pNC biosensors[2].
Suc-Ala-Ala-Pro-Val-AMC is cleaved by elastase released from human blood PMNs incubated with LDL, with higher LDL concentrations correlating with greater elastase activity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 113277-37-5
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Appearance Solid
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Molecular Weight 613.66
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Formula C30H39N5O9
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Color White to off-white
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Sequence
{Suc}-Ala-Ala-Pro-Val-{AMC}
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Sequence Shortening
{Suc}-AAPV-{AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (162.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Fontenot KR, et al. Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design. J Biomater Appl. 2017;32(5):622-637. [Content Brief]
[3]. Polacek D, et al. In vitro proteolysis of human plasma low density lipoproteins by an elastase released from human blood polymorphonuclear cells. J Biol Chem. 1986;261(5):2057-2063. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6296 mL | 8.1478 mL | 16.2957 mL | 40.7392 mL |
| 5 mM | 0.3259 mL | 1.6296 mL | 3.2591 mL | 8.1478 mL | |
| 10 mM | 0.1630 mL | 0.8148 mL | 1.6296 mL | 4.0739 mL | |
| 15 mM | 0.1086 mL | 0.5432 mL | 1.0864 mL | 2.7159 mL | |
| 20 mM | 0.0815 mL | 0.4074 mL | 0.8148 mL | 2.0370 mL | |
| 25 mM | 0.0652 mL | 0.3259 mL | 0.6518 mL | 1.6296 mL | |
| 30 mM | 0.0543 mL | 0.2716 mL | 0.5432 mL | 1.3580 mL | |
| 40 mM | 0.0407 mL | 0.2037 mL | 0.4074 mL | 1.0185 mL | |
| 50 mM | 0.0326 mL | 0.1630 mL | 0.3259 mL | 0.8148 mL | |
| 60 mM | 0.0272 mL | 0.1358 mL | 0.2716 mL | 0.6790 mL | |
| 80 mM | 0.0204 mL | 0.1018 mL | 0.2037 mL | 0.5092 mL | |
| 100 mM | 0.0163 mL | 0.0815 mL | 0.1630 mL | 0.4074 mL |
Suc-Ala-Ala-Pro-Val-AMC Related Classifications
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