TAMRA-PEG4-Alkyne

Name: TAMRA-PEG4-Alkyne
Brand: MedChemExpress
SKU: HY-120666
Rating: 4.5 (1 reviews)

Cat. No.: HY-120666 Purity: 98.67%: Data Sheet
COA

SDS
Handling Instructions
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TAMRA-PEG4-Alkyne is a dye derivative of TAMRA (HY-135640) containing 4 PEG units. TAMRA-PEG4-Alkyne contains Alkyne groups and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups (Ex/Em = 553/575 nm).

For research use only. We do not sell to patients.

TAMRA-PEG4-Alkyne Chemical Structure

CAS No. : 1225057-68-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
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Description

IC₅₀ & Target

PEGs

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Gel Fluorescence Analysis of Probe-Modified Proteins.
1.1 Incubate BSA or proteins extracted from MCF7 cells with sulfonated probe at 25 °C for 3 hours.
1.2 Add freshly premixed click chemistry reaction mixture (50 μM TAMRA-PEG4-Alkyne, 100 μM TBTA, 1 mM TCEP, and 1 mM CuSO4) to the above mixture.
1.3 Incubate at room temperature for 1 hour.
1.4 Then add 4× SDS-PAGE loading buffer and boil at 95°C for 10 minutes.
1.5 Analyze the samples using a 12% SDS-PAGE gel (polyacrylamide gel electrophoresis).

2. Cellular Imaging.
2.1 MCF7 cells were washed with PBS and pretreated with a sulfonium-based probe for 1 hour.
2.2 They were washed three times with PBS.
2.3 Then, they were fixed with 300 μL of 4% formaldehyde PBS solution for 15 minutes, followed by permeabilization with 300 μL of 0.1% Triton X-100 PBS solution for 15 minutes.
2.4 Freshly premixed click chemistry reaction mixture (10 μM TAMRA-PEG4-Alkyne (DMSO stock solution), 10 μM TBTA, 0.1 mM TCEP, and 0.1 mM CuSO4) was added to the mixture.
2.5 Incubation continued at room temperature for 1 hour.
2.6 The cells were washed three times with PBS, followed by one wash with 0.1% Tween PBS solution.
2.7 Finally, the cells were stained with 4′,6-diamidinyl-2-phenylindole (DAPI) for imaging.
2.8 The cells were observed using a confocal microscope or a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

643.73

Formula

C₃₆H₄₁N₃O₈

CAS No.

1225057-68-0

Appearance

Solid

Color

Brown to reddish brown

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(C(NCCOCCOCCOCCOCC#C)=O)C=C4C([O-])=O)=C2C=C1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (155.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5534 mL	7.7672 mL	15.5345 mL
5 mM	0.3107 mL	1.5534 mL	3.1069 mL
10 mM	0.1553 mL	0.7767 mL	1.5534 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.67%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (247 KB) HNMR (316 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Wang R, et al. Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5534 mL	7.7672 mL	15.5345 mL	38.8362 mL
	5 mM	0.3107 mL	1.5534 mL	3.1069 mL	7.7672 mL
	10 mM	0.1553 mL	0.7767 mL	1.5534 mL	3.8836 mL
	15 mM	0.1036 mL	0.5178 mL	1.0356 mL	2.5891 mL
	20 mM	0.0777 mL	0.3884 mL	0.7767 mL	1.9418 mL
	25 mM	0.0621 mL	0.3107 mL	0.6214 mL	1.5534 mL
	30 mM	0.0518 mL	0.2589 mL	0.5178 mL	1.2945 mL
	40 mM	0.0388 mL	0.1942 mL	0.3884 mL	0.9709 mL
	50 mM	0.0311 mL	0.1553 mL	0.3107 mL	0.7767 mL
	60 mM	0.0259 mL	0.1295 mL	0.2589 mL	0.6473 mL
	80 mM	0.0194 mL	0.0971 mL	0.1942 mL	0.4855 mL
	100 mM	0.0155 mL	0.0777 mL	0.1553 mL	0.3884 mL