TCEP-d12 hydrochloride
TCEP-d12 (hydrochloride) (Tris(2-?carboxyethyl)?phosphine hydrochloride-d12) is deuterium labeled TCEP (hydrochloride). TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry.
For research use only. We do not sell to patients.
- CAS No.: 1189959-10-1
- Formula: C9H4D12ClO6P
- Molecular Weight:298.72
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 1189959-10-1
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Unlabeled Cas 51805-45-9
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Molecular Weight 298.72
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Formula C9H4D12ClO6P
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SMILES
OC(C([2H])([2H])C([2H])([2H])P(C([2H])([2H])C([2H])([2H])C(O)=O)C([2H])([2H])C([2H])([2H])C(O)=O)=O.Cl
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Synonyms
Tris(2-carboxyethyl)phosphine hydrochloride-d12
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (334.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (167.38 mM; ultrasonic and adjust pH to 7 with NaOH)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [Content Brief]
[2]. Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994;220(1):5-10. [Content Brief]
[3]. Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14(1):7-16. [Content Brief]
[4]. Duchardt F, et al. A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. J Biol Chem. 2009 Dec 25;284(52):36099-108. [Content Brief]
[5]. Sequeira MA, et al. Modulating amyloid fibrillation in a minimalist model peptide by intermolecular disulfide chemical reduction. Phys Chem Chem Phys. 2019 Jun 5;21(22):11916-11923. [Content Brief]
[6]. Wu R, et al. Effects of Small Molecules on DNA Adsorption by Gold Nanoparticles and a Case Study of Tris(2-carboxyethyl)phosphine (TCEP). Langmuir. 2019 Oct 15;35(41):13461-13468. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3476 mL | 16.7381 mL | 33.4762 mL | 83.6904 mL |
| 5 mM | 0.6695 mL | 3.3476 mL | 6.6952 mL | 16.7381 mL | |
| 10 mM | 0.3348 mL | 1.6738 mL | 3.3476 mL | 8.3690 mL | |
| 15 mM | 0.2232 mL | 1.1159 mL | 2.2317 mL | 5.5794 mL | |
| 20 mM | 0.1674 mL | 0.8369 mL | 1.6738 mL | 4.1845 mL | |
| 25 mM | 0.1339 mL | 0.6695 mL | 1.3390 mL | 3.3476 mL | |
| 30 mM | 0.1116 mL | 0.5579 mL | 1.1159 mL | 2.7897 mL | |
| 40 mM | 0.0837 mL | 0.4185 mL | 0.8369 mL | 2.0923 mL | |
| 50 mM | 0.0670 mL | 0.3348 mL | 0.6695 mL | 1.6738 mL | |
| 60 mM | 0.0558 mL | 0.2790 mL | 0.5579 mL | 1.3948 mL | |
| 80 mM | 0.0418 mL | 0.2092 mL | 0.4185 mL | 1.0461 mL | |
| 100 mM | 0.0335 mL | 0.1674 mL | 0.3348 mL | 0.8369 mL |
TCEP-d12 hydrochloride Related Classifications
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