TMPD dihydrochloride

Name: TMPD dihydrochloride
Brand: MedChemExpress
SKU: HY-W012145
Rating: 4.5 (1 reviews)

Cat. No.: HY-W012145 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TMPD dihydrochloride, a readily oxidizable compound, is an enzymatically convert redox active substrate molecule. TMPD dihydrochloride is also an electron donor and serves as a reducing cosubstrate for heme peroxidases. TMPD dihydrochloride is also a complex IV substrate.

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TMPD dihydrochloride Chemical Structure

CAS No. : 637-01-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 25	In-stock	Estimated Time of Arrival: December 31
5 g	USD 70	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

TMPD dihydrochloride, a readily oxidizable compound, is an enzymatically convert redox active substrate molecule. TMPD dihydrochloride is also an electron donor and serves as a reducing cosubstrate for heme peroxidases^[1]^[2]. TMPD dihydrochloride is also a complex IV substrate^[3].

In Vitro

TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. The concept of TMPD oxidation by microorganisms is widely used and is known as a successful colorimetric indicator for bacterial oxidases, as the radical cation TMPD+˙, formed by oxidation, shows a characteristic deep blue colour. The electrochemical recognition of the TMPD oxidation by bacterial oxidases can be applied to a variety of pathogens^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

237.17

Formula

C₁₀H₁₈Cl₂N₂

CAS No.

637-01-4

Appearance

Solid

SMILES

CN(C)C1=CC=C(N(C)C)C=C1.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (421.64 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (70.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2164 mL	21.0819 mL	42.1638 mL
5 mM	0.8433 mL	4.2164 mL	8.4328 mL
10 mM	0.4216 mL	2.1082 mL	4.2164 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (7.04 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (421.64 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (231 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. S Kuss, et al. Electrochemical recognition and quantification of cytochrome c expression in Bacillus subtilis and aerobe/anaerobe Escherichia coli using N, N, N', N'-tetramethyl- para-phenylene-diamine (TMPD). Chem Sci. 2017 Nov 1;8(11):7682-7688. [Content Brief]

[2]. Nenad Petrovic, et al. Using N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) to assay cyclooxygenase activity in vitro. Methods Mol Biol. 2010;594:129-40. [Content Brief]

[3]. Franco A Rossato, et al. Fatty acid synthase inhibitors induce apoptosis in non-tumorigenic melan-a cells associated with inhibition of mitochondrial respiration. PLoS One. 2014 Jun 25;9(6):e101060. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.2164 mL	21.0819 mL	42.1638 mL	105.4096 mL
	5 mM	0.8433 mL	4.2164 mL	8.4328 mL	21.0819 mL
	10 mM	0.4216 mL	2.1082 mL	4.2164 mL	10.5410 mL
	15 mM	0.2811 mL	1.4055 mL	2.8109 mL	7.0273 mL
	20 mM	0.2108 mL	1.0541 mL	2.1082 mL	5.2705 mL
	25 mM	0.1687 mL	0.8433 mL	1.6866 mL	4.2164 mL
	30 mM	0.1405 mL	0.7027 mL	1.4055 mL	3.5137 mL
	40 mM	0.1054 mL	0.5270 mL	1.0541 mL	2.6352 mL
	50 mM	0.0843 mL	0.4216 mL	0.8433 mL	2.1082 mL
	60 mM	0.0703 mL	0.3514 mL	0.7027 mL	1.7568 mL
H₂O	80 mM	0.0527 mL	0.2635 mL	0.5270 mL	1.3176 mL
H₂O	100 mM	0.0422 mL	0.2108 mL	0.4216 mL	1.0541 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.