TNP-GTP tetrasodium
TNP-GTP tetrasodium is a fluorescently labeled GTP (HY-113225) derivative. TNP-GTP tetrasodium can inhibit glutamate dehydrogenase (Ki = 2.7 μM). TNP-GTP tetrasodium is an antagonist of the purinergic P2X2 and P2X2/3 receptors with IC50 values of 0.4 and 1.2 nM, respectively. TNP-GTP tetrasodium also inhibit rat soluble guanylyl cyclase (Ki = 11 nM).
For research use only. We do not sell to patients.
- Formula: C16H13N8Na4O20P3
- Molecular Weight:822.20
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All P2X Receptor Isoforms
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Biological Activity
Description
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P2X2 Receptor |
P2X3 Receptor |
Chemical Information
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Molecular Weight 822.20
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Formula C16H13N8Na4O20P3
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SMILES
O=C1C2=C(N([C@H]3[C@H](OC4(C([N+]([O-])=O)=CC([N+]([O-])=O)C=C4[N+]([O-])=O)O5)[C@H]5[C@@H](COP(OP(OP(O[Na])(O[Na])=O)(O[Na])=O)(O[Na])=O)O3)C=N2)N=C(N)N1
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Synonyms
TNP-Guanosine 5'-triphosphate tetrasodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
[1]. Hiratsuka T. A chromophoric and fluorescent analog of GTP, 2',3'-O-(2,4,6-trinitrocyclohexadienylidene)-GTP, as a spectroscopic probe for the GTP inhibitory site of liver glutamate dehydrogenase. J Biol Chem. 1985 Apr 25;260(8):4784-90. [Content Brief]
[2]. Virginio C, et al. Trinitrophenyl-substituted nucleotides are potent antagonists selective for P2X1, P2X3, and heteromeric P2X2/3 receptors. Mol Pharmacol. 1998 Jun;53(6):969-73. [Content Brief]
[3]. Dove S, et al. Structure/activity relationships of (M)ANT- and TNP-nucleotides for inhibition of rat soluble guanylyl cyclase α1β1. Mol Pharmacol. 2014 Apr;85(4):598-607. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TNP-GTP tetrasodium Related Classifications
HY-137609A Related Classifications
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