Tricaine methanesulfonate
Based on 27 publication(s) in Google Scholar
Tricaine methanesulfonate (MS-222) is common used to immobilize fish for marking or transport and to suppress sensory systems during invasive procedures.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 886-86-2
- Formula: C10H15NO5S
- Molecular Weight:261.30
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tricaine methanesulfonate
More- Adv Sci (Weinh). 2024 Dec;11(47):e2406026. [Abstract]
- Food Chem. 2026 May 1:510:148652. [Abstract]
- Int J Biol Macromol. 2025 Aug;319(Pt 4):145467. [Abstract]
- J Agric Food Chem. 2026 Jul 8;74(26):20662-20673. [Abstract]
- Biomater Adv. 2026 Jun:183:214773. [Abstract]
- Life Sci. 2023 Sep 15:329:121956. [Abstract]
- Int J Mol Sci. 2024 Dec 23;25(24):13726. [Abstract]
- Int J Mol Sci. 2023 Apr 20;24(8):7576. [Abstract]
- RSC Pharm. 2026 Mar 19.
- Chem Biol Interact. 2026 Apr 25:429:111957. [Abstract]
- Chem Biol Interact. 2026 Mar 1:426:111911. [Abstract]
- Sci Rep. 2026 Apr 1;16(1):14708. [Abstract]
- Pestic Biochem Physiol. 2026 Jun:221:107178. [Abstract]
- Aquat Toxicol. 2026 May:294:107792. [Abstract]
- Aquac Nutr. 2025 Oct 26:2025:2234393. [Abstract]
- Aquaculture. 2026 Apr 29.
- Aquaculture. 2026 Jan 1.
- Aquaculture. 2025 Apr 30.
- Cell Stress Chaperones. 2025 Sep 17:100112. [Abstract]
- Water. 2022, 14(2), 160.
- Biol Reprod. 2025 Jun 15;112(6):1185-1199. [Abstract]
- J Appl Toxicol. 2025 Nov 30. [Abstract]
- Comp Biochem Physiol Part D Genomics Proteomics. 2026 Mar:57:101687. [Abstract]
- Fishes. 2025 Aug 5.
- Comp Biochem Physiol B Biochem Mol Biol. 2025 Jan:275:111021. [Abstract]
- bioRxiv. 2025 Jun 09.
- Biomed Pharmacother. 2023 Oct:166:115397. [Abstract]
Biological Activity
Tricaine methanesulfonate in powder form easily can become airborne and could become hazardous to the handler[1].
Tricaine methanesulfonate (TMS) solution is photosensitive (its color changes with light exposure), although the color change does not necessarily indicate significant activity decrease and thus color should not be used to gauge degradation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 886-86-2
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Appearance Solid
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Molecular Weight 261.30
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Formula C10H15NO5S
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Color White to off-white
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SMILES
O=C(OCC)C1=CC=CC(N)=C1.CS(=O)(O)=O
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Synonyms
MS-222
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (27)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption. [Abstract]2024 Dec;11(47):e2406026. PMID: 39494721 -
Food Chem
Food-grade fish oil supplementation promotes membrane and redox stability during cryopreservation of aquatic biomaterials. [Abstract]2026 May 1:510:148652. PMID: 41785761 -
Int J Biol Macromol
Multipotent anti-aging recombinant human elastin: Counteracting zebrafish caudal fin shrinkage and D-galactose-induced intrinsic skin aging in mice. [Abstract]2025 Aug;319(Pt 4):145467. PMID: 40571014 -
J Agric Food Chem
Enantioselective Neurotoxicity of Pydiflumetofen in Zebrafish Embryos: R-Enantiomer Disrupts Neurodevelopment via Suppression of Wnt/β-Catenin Signaling. [Abstract]2026 Jul 8;74(26):20662-20673. PMID: 42345402 -
Biomater Adv
A biointerface-engineered gold nanocluster platform for icariin delivery: Dual-pathway modulation of osteoblast and osteoclast dynamics in osteoporosis therapy. [Abstract]2026 Jun:183:214773. PMID: 41691763 -
Life Sci
2023 Sep 15:329:121956. PMID: 37473802 -
Int J Mol Sci
Genomic Analysis and Functional Validation of Bidirectional Promoters in Medaka (Oryzias latipes). [Abstract]2024 Dec 23;25(24):13726. PMID: 39769487 -
Int J Mol Sci
50 Hz Magnetic Field Exposure Inhibited Spontaneous Movement of Zebrafish Larvae through ROS-Mediated syn2a Expression. [Abstract]2023 Apr 20;24(8):7576. PMID: 37108734 -
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Chem Biol Interact
Cyflumetofen induces hepatic steatosis and disrupts lipid metabolism in zebrafish larvae. [Abstract]2026 Apr 25:429:111957. PMID: 41654006 -
Chem Biol Interact
Cyflumetofen disrupts the blood-brain barrier and induces neurotoxicity in zebrafish larvae. [Abstract]2026 Mar 1:426:111911. PMID: 41539556 -
Sci Rep
Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. [Abstract]2026 Apr 1;16(1):14708. PMID: 41922548 -
Pestic Biochem Physiol
Avermectin disrupts blood-brain barrier integrity in zebrafish larvae via the Wnt/β-catenin signaling pathway. [Abstract]2026 Jun:221:107178. PMID: 42264772 -
Aquat Toxicol
Exposure to magnesium oxide nanoparticles induces mitochondrial dysfunction and apoptosis in fish Megalobrama amblycephala by disrupting magnesium homeostasis. [Abstract]2026 May:294:107792. PMID: 41850154 -
Aquac Nutr
Dietary Fermented Chicory Root Waste Modulates Growth, Chemical Composition, Lipid Metabolism, and Intestinal Barrier Pathways in Common Carp (Cyprinus carpio L.) Fed With High-Fat Diets. [Abstract]2025 Oct 26:2025:2234393. PMID: 41185752 -
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Cell Stress Chaperones
The role of Atp2a2-mediated calcium imbalance and endoplasmic reticulum stress in hydrocortisone-induced neurotoxicity. [Abstract]2025 Sep 17:100112. PMID: 40972936 -
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Biol Reprod
Morphological characterization of spermatogenesis and spermatogonial stem cells in Larimichthys crocea, a seasonal breeding teleost†. [Abstract]2025 Jun 15;112(6):1185-1199. PMID: 40207864 -
J Appl Toxicol
Hepatotoxicity Mechanisms of Sirolimus and Everolimus Unveiled Through Metabolomics and Transcriptomics Analysis. [Abstract]2025 Nov 30. PMID: 41320894 -
Comp Biochem Physiol Part D Genomics Proteomics
The sex-specific transcriptomic patterns in hypothalamic-pituitary-gonadal axis of Dabry's sturgeon (Acipenser dabryanus). [Abstract]2026 Mar:57:101687. PMID: 41252948 -
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Comp Biochem Physiol B Biochem Mol Biol
Comparative study on the transcriptional activities of grass carp (Ctenopharyngodon idellus) peroxisome proliferator-activated receptors induced by different fatty acids. [Abstract]2025 Jan:275:111021. PMID: 39151662 -
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Biomed Pharmacother
Toxic effects of sirolimus and everolimus on the development and behavior of zebrafish embryos. [Abstract]2023 Oct:166:115397. PMID: 37659200
Solvent & Solubility
H2O : ≥ 100 mg/mL (382.70 mM)
DMSO : 100 mg/mL (382.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (19.14 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (19.14 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.8270 mL | 19.1352 mL | 38.2703 mL | 95.6758 mL |
| 5 mM | 0.7654 mL | 3.8270 mL | 7.6541 mL | 19.1352 mL | |
| 10 mM | 0.3827 mL | 1.9135 mL | 3.8270 mL | 9.5676 mL | |
| 15 mM | 0.2551 mL | 1.2757 mL | 2.5514 mL | 6.3784 mL | |
| 20 mM | 0.1914 mL | 0.9568 mL | 1.9135 mL | 4.7838 mL | |
| 25 mM | 0.1531 mL | 0.7654 mL | 1.5308 mL | 3.8270 mL | |
| 30 mM | 0.1276 mL | 0.6378 mL | 1.2757 mL | 3.1892 mL | |
| 40 mM | 0.0957 mL | 0.4784 mL | 0.9568 mL | 2.3919 mL | |
| 50 mM | 0.0765 mL | 0.3827 mL | 0.7654 mL | 1.9135 mL | |
| 60 mM | 0.0638 mL | 0.3189 mL | 0.6378 mL | 1.5946 mL | |
| 80 mM | 0.0478 mL | 0.2392 mL | 0.4784 mL | 1.1959 mL | |
| 100 mM | 0.0383 mL | 0.1914 mL | 0.3827 mL | 0.9568 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Tricaine methanesulfonate Related Classifications
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