Tubulin inhibitor 12
Tubulin inhibitor 12 (Hit 9) is a novel tubulin inhibitor (IC50=25.3 μM). Tubulin inhibitor 12 shows anti-tumor activity and anti-proliferative activity.
For research use only. We do not sell to patients.
- CAS No.: 92575-00-3
- Formula: C24H20N2O
- Molecular Weight:352.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Tubulin inhibitor 12 (0-100 μM; 72 h) shows moderate and broad-spectrum anti-proliferative activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepG2, A549, and HCT116 cells
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Concentration:0-100 μM
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Incubation Time:72 hours
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Result:Showed IC50 values of 49.13, 52.84, and 70.10 μM for HepG2, A549, and HCT116 cells, respectively.
Chemical Information
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CAS No. 92575-00-3
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Molecular Weight 352.43
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Formula C24H20N2O
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SMILES
CN(C(C1=CC(C2=CC=CC=C2)=NC3=CC=CC=C31)=O)CC4=CC=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Tubulin inhibitor 12 Related Classifications
HY-143250 Related Classifications
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