Tyrosinase-related Protein 2 (TRP-2) (181-188)
Based on 1 Customer Validation
Tyrosinase-related Protein 2 (TRP-2) (181-188) is a peptide epitope of the TRP-2 protein that conforms to the MHC class I H2-Kb binding motif. It is the major reactive epitope within TRP-2 recognized by anti-B16 CTLs, induces TRP-2-specific CTL and CD8+ T cell responses, and mediates antibody-dependent antitumor effects against lung metastasis. Tyrosinase-related Protein 2 (TRP-2) (181-188) is used in melanoma research.
For research use only. We do not sell to patients.
- Purity : 98.52%
- CAS No.: 187671-49-4
- Formula: C58H73N9O12
- Molecular Weight:1088.25
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
TRP-2 181-188 (10 μg/mL; 90 min) is the major reactive epitope within TRP-2 recognized by anti-B16 line A CTLs when presented by MC38 cells, stimulating 6.0 U/mL/24 h of IFN-γ release[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Tyrosinase‑related Protein 2 (TRP‑2) (181‑188) peptide co‑injected with IC31 (100 µg/mouse TRP‑2181‑188 peptide; 100 nmol/mouse KLKL5KLK; 5 nmol/mouse ODN1a; single intrapad injection) induces robust peptide‑specific type 1 cellular immune response with approximately 155 IFN‑γ‑producing cells per 106 splenocytes[3].
Tyrosinase-related Protein 2 (TRP-2) (181-188) peptide with a single injection of IC31 (100 µg/mouse TRP-2181-188 peptide; 100 nmol/mouse KLKL5KLK; 5 nmol/mouse ODN1a) induces potent TRP-2181-188-specific CTL effector cells, producing 69.2% specific lysis of target cells in vivo[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (female, 6-12 weeks old)[1]
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Dosage:1-2 × 106 CTLs; 6 × 104 IU IL-2
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Administration:i.v. (single dose on day 3); i.p. (three times per day for 3 days)
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Result:Showed a highly significant effect against 3-day-old B16 lung metastases when administered intravenously in combination with low-dose IL-2.
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Animal Model:C57BL/6[2]
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Dosage:50 μl
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Administration:i.v.; at day 0, 3, and 6
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Result:Delayed tumor growth when compared with sera from mice immunized with VRP expressing other antigens.
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Animal Model:C57BL/6 (H-2b; female; 6-10 weeks old)[3]
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Dosage:100 µg/mouse (TRP-2181-188 peptide); 100 nmol/mouse (KLKL5KLK); 5 nmol/mouse (ODN1a)
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Administration:Intrafootpad; single injection
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Result:Induced approximately 155 IFN-γ-producing cells per 106 splenocytes when co-injected with IC31.
Induced no peptide-specific IFN-γ-producing cells when injected alone or with KLKL5KLK or ODN1a alone.
Induced no peptide-specific IL-4 production.
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Animal Model:C57BL/6 (H-2b; female; 6-10 weeks old)[3]
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Dosage:100 µg/mouse (TRP-2181-188 peptide); 100 nmol/mouse (KLKL5KLK); 5 nmol/mouse (ODN1a)
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Administration:Injection; single dose
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Result:Induced 69.2% antigen-specific lysis of target cells in vivo when co-injected with IC31.
Induced no cytotoxic activity in naïve mice (21.3%).
Induced no cytotoxic activity in mice vaccinated with peptide alone (18.9%).
Unspecific lysis of target cells pulsed with irrelevant antigen was not higher than 15%.
Chemical Information
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CAS No. 187671-49-4
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Appearance Solid
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Molecular Weight 1088.25
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Formula C58H73N9O12
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Color White to off-white
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Sequence
Val-Tyr-Asp-Phe-Phe-Val-Trp-Leu
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Sequence Shortening
VYDFFVWL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : ≥ 12.5 mg/mL (11.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (291 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9189 mL | 4.5945 mL | 9.1891 mL | 22.9727 mL |
| 5 mM | 0.1838 mL | 0.9189 mL | 1.8378 mL | 4.5945 mL | |
| 10 mM | 0.0919 mL | 0.4595 mL | 0.9189 mL | 2.2973 mL |
Tyrosinase-related Protein 2 (TRP-2) (181-188) Related Classifications
HY-P2527 Related Classifications
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