UBA6-IN-1 hydrochloride
Based on 1 Customer Validation
UBA6-IN-1 hydrochloride is a UBA6/UBE1L2 inhibitor (IC50 ≤50 nM) that acts by reducing the level of squalene epoxidase (SQLE). UBA6-IN-1 hydrochloride affects SREBP-dependent lipid metabolism processes and downstream cholesteryl ester transport, and can be used for cancer research.
For research use only. We do not sell to patients.
- CAS No.: 1557138-61-0
- Formula: C20H22ClN5O4S
- Molecular Weight:463.94
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
UBA6-IN-1 hydrochloride (I-01) potently inhibits Uba6 enzyme activity, achieving 87% inhibition at 0.123 μM[1].
UBA6-IN-1 (10 μM; 6 h) hydrochloride alters SREBP-dependent lipid metabolism in WSU-DLCL2 cells when treated at 10 μM for 6 hours, as indicated by reduced SQLE protein levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1557138-61-0
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Appearance Solid
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Molecular Weight 463.94
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Formula C20H22ClN5O4S
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SMILES
O[C@H]1C[C@H](N(C=C2)C3=C2C(C4=CC5=CC=CC=C5N4)=NC=N3)C[C@H]1COS(N)(=O)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (215.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1555 mL | 10.7773 mL | 21.5545 mL | 53.8863 mL |
| 5 mM | 0.4311 mL | 2.1555 mL | 4.3109 mL | 10.7773 mL | |
| 10 mM | 0.2155 mL | 1.0777 mL | 2.1555 mL | 5.3886 mL | |
| 15 mM | 0.1437 mL | 0.7185 mL | 1.4370 mL | 3.5924 mL | |
| 20 mM | 0.1078 mL | 0.5389 mL | 1.0777 mL | 2.6943 mL | |
| 25 mM | 0.0862 mL | 0.4311 mL | 0.8622 mL | 2.1555 mL | |
| 30 mM | 0.0718 mL | 0.3592 mL | 0.7185 mL | 1.7962 mL | |
| 40 mM | 0.0539 mL | 0.2694 mL | 0.5389 mL | 1.3472 mL | |
| 50 mM | 0.0431 mL | 0.2155 mL | 0.4311 mL | 1.0777 mL | |
| 60 mM | 0.0359 mL | 0.1796 mL | 0.3592 mL | 0.8981 mL | |
| 80 mM | 0.0269 mL | 0.1347 mL | 0.2694 mL | 0.6736 mL | |
| 100 mM | 0.0216 mL | 0.1078 mL | 0.2155 mL | 0.5389 mL |
UBA6-IN-1 hydrochloride Related Classifications
HY-153774A Related Classifications
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