UBA6-IN-1
UBA6-IN-1 is a UBA6/UBE1L2 inhibitor (IC50 ≤50 nM) that acts by reducing the level of squalene epoxidase (SQLE). UBA6-IN-1 affects SREBP-dependent lipid metabolism processes and downstream cholesteryl ester transport, and can be used for cancer research.
For research use only. We do not sell to patients.
- CAS No.: 1557137-61-7
- Formula: C20H21N5O4S
- Molecular Weight:427.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
UBA6-IN-1 (I-01) potently inhibits Uba6 enzyme activity, achieving 87% inhibition at 0.123 μM[1].
UBA6-IN-1 (10 μM; 6 h) alters SREBP-dependent lipid metabolism in WSU-DLCL2 cells when treated at 10 μM for 6 hours, as indicated by reduced SQLE protein levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1557137-61-7
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Molecular Weight 427.48
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Formula C20H21N5O4S
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SMILES
NS(OC[C@@H]1C[C@H](C[C@@H]1O)N2C3=NC=NC(C4=CC5=CC=CC=C5N4)=C3C=C2)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (233.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3393 mL | 11.6965 mL | 23.3929 mL | 58.4823 mL |
| 5 mM | 0.4679 mL | 2.3393 mL | 4.6786 mL | 11.6965 mL | |
| 10 mM | 0.2339 mL | 1.1696 mL | 2.3393 mL | 5.8482 mL | |
| 15 mM | 0.1560 mL | 0.7798 mL | 1.5595 mL | 3.8988 mL | |
| 20 mM | 0.1170 mL | 0.5848 mL | 1.1696 mL | 2.9241 mL | |
| 25 mM | 0.0936 mL | 0.4679 mL | 0.9357 mL | 2.3393 mL | |
| 30 mM | 0.0780 mL | 0.3899 mL | 0.7798 mL | 1.9494 mL | |
| 40 mM | 0.0585 mL | 0.2924 mL | 0.5848 mL | 1.4621 mL | |
| 50 mM | 0.0468 mL | 0.2339 mL | 0.4679 mL | 1.1696 mL | |
| 60 mM | 0.0390 mL | 0.1949 mL | 0.3899 mL | 0.9747 mL | |
| 80 mM | 0.0292 mL | 0.1462 mL | 0.2924 mL | 0.7310 mL | |
| 100 mM | 0.0234 mL | 0.1170 mL | 0.2339 mL | 0.5848 mL |
UBA6-IN-1 Related Classifications
HY-153774 Related Classifications
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