PROT-IN-1
Based on 1 Customer Validation
PROT-IN-1 is a PROT inhibitor (IC50: 1.48 μM). PROT-IN-1 is applicable to research related to cognitive impairment.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 329935-45-7
- Formula: C23H22N2O
- Molecular Weight:342.43
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
PROT 1.48 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-1 | IC50 |
1.48 μM
Compound: 2
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Inhibition of human high-affinity L-proline transporter expressed in COS1 cells by [3H]proline uptake assay
Inhibition of human high-affinity L-proline transporter expressed in COS1 cells by [3H]proline uptake assay
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[PMID: 25037917] |
In Vitro
PROT-IN-1 (Compound 2) inhibits PROT-mediated 3H-proline uptake in PROT-expressing COS1 cells with an IC50 of 1.48 μM, and exhibits metabolic stability in mouse liver S9 fractions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 329935-45-7
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Appearance Solid
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Molecular Weight 342.43
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Formula C23H22N2O
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Color White to off-white
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SMILES
O=C(N1CCN(CC1)C2=CC=CC=C2)C3=CC=C(C4=CC=CC=C4)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (73.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9203 mL | 14.6015 mL | 29.2030 mL | 73.0076 mL |
| 5 mM | 0.5841 mL | 2.9203 mL | 5.8406 mL | 14.6015 mL | |
| 10 mM | 0.2920 mL | 1.4602 mL | 2.9203 mL | 7.3008 mL | |
| 15 mM | 0.1947 mL | 0.9734 mL | 1.9469 mL | 4.8672 mL | |
| 20 mM | 0.1460 mL | 0.7301 mL | 1.4602 mL | 3.6504 mL | |
| 25 mM | 0.1168 mL | 0.5841 mL | 1.1681 mL | 2.9203 mL | |
| 30 mM | 0.0973 mL | 0.4867 mL | 0.9734 mL | 2.4336 mL | |
| 40 mM | 0.0730 mL | 0.3650 mL | 0.7301 mL | 1.8252 mL | |
| 50 mM | 0.0584 mL | 0.2920 mL | 0.5841 mL | 1.4602 mL | |
| 60 mM | 0.0487 mL | 0.2434 mL | 0.4867 mL | 1.2168 mL |
PROT-IN-1 Related Classifications
HY-153763 Related Classifications
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