Wnt/β-catenin agonist 3

Name: Wnt/β-catenin agonist 3
Brand: MedChemExpress
SKU: HY-148055
Rating: 4.5 (1 reviews)

Cat. No.: HY-148055 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Wnt/β-catenin agonist 3 (compound 98) is a Wnt/β-catenin signalling pathway agonist. Wnt/β-catenin agonist 3 can be used for the research of osteoporosis.

For research use only. We do not sell to patients.

Wnt/β-catenin agonist 3 Chemical Structure

CAS No. : 912790-59-1

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 93	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 93	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Wnt/β-catenin agonist 3:

Wnt/β-catenin agonist 3 hydrochloride In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Wnt/β-catenin agonist 3 (compound 98) is a Wnt/β-catenin signalling pathway agonist. Wnt/β-catenin agonist 3 can be used for the research of osteoporosis^[1].

In Vitro

Wnt/β-catenin agonist 3 (compound 98; 24 h; HEK293 and SW480 cells) has 54% cell activity at 120 μM concentration compared with the positive control group LiCl (20mM)^[1].
Wnt/β-catenin agonist 3 (30 and 60 μM; 24 h; HEK293 cells) is an activator for β-Catenin and deposits β-catenin^[1].
Wnt/β-catenin agonist 3 (11 μM; 4 d) induces differentiation of ST2 cell line into osteoblasts and calcium deposition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK293 cells
Concentration:	30 and 60 μM
Incubation Time:	24 hours
Result:	Deposited β-catenin in a dose-dependent pattern within cells.

Molecular Weight

330.77

Formula

C₁₆H₁₅ClN₄O₂

CAS No.

912790-59-1

Appearance

Solid

Color

White to light brown

SMILES

O=C(C1=NOC(C2=CC=C(Cl)C=C2)=C1)NCCCN3C=CN=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (377.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0232 mL	15.1162 mL	30.2325 mL
5 mM	0.6046 mL	3.0232 mL	6.0465 mL
10 mM	0.3023 mL	1.5116 mL	3.0232 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (246 KB) HNMR (268 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Cho JW, et al. Isoxazole derivatives and use thereof. WO2007078113A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0232 mL	15.1162 mL	30.2325 mL	75.5812 mL
	5 mM	0.6046 mL	3.0232 mL	6.0465 mL	15.1162 mL
	10 mM	0.3023 mL	1.5116 mL	3.0232 mL	7.5581 mL
	15 mM	0.2015 mL	1.0077 mL	2.0155 mL	5.0387 mL
	20 mM	0.1512 mL	0.7558 mL	1.5116 mL	3.7791 mL
	25 mM	0.1209 mL	0.6046 mL	1.2093 mL	3.0232 mL
	30 mM	0.1008 mL	0.5039 mL	1.0077 mL	2.5194 mL
	40 mM	0.0756 mL	0.3779 mL	0.7558 mL	1.8895 mL
	50 mM	0.0605 mL	0.3023 mL	0.6046 mL	1.5116 mL
	60 mM	0.0504 mL	0.2519 mL	0.5039 mL	1.2597 mL
	80 mM	0.0378 mL	0.1890 mL	0.3779 mL	0.9448 mL
	100 mM	0.0302 mL	0.1512 mL	0.3023 mL	0.7558 mL