10-Formylfolic acid
Based on 1 Customer Validation
10-Formylfolic acid is a potent inhibitor of dihydrofolate reductase, with an IC50 of 80 nM against the rat enzyme. 10-Formylfolic acid also acts as an orally active folate source and a growth factor for specific bacteria. 10-Formylfolic acid supports growth and increases plasma folate levels; its absorption and metabolism patterns are similar to those of folic acid, but its liver retention is slightly higher. It exhibits high folate activity in microbial assays, serves as a growth factor for Enterococcus faecalis and Lactobacillus casei, aids folate nutrient supply, and may exert cell regulatory functions.
For research use only. We do not sell to patients.
- Purity : 94.49%
- CAS No.: 134-05-4
- Formula: C20H19N7O7
- Molecular Weight:469.41
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-14521
LometrexolGARFT Inhibitor -
HY-124350R
10-Formylfolic acid (Standard)Reference Standard -
HY-10820
Pemetrexed -
HY-137439
FanotaprimDHFR Inhibitor -
HY-130251
DS18561882MTHFD2 Inhibitor -
HY-13664
Folinic acid calciumBiological Folic Acid -
HY-17556
Folinic acidBiological Folic Acid -
HY-101943
LY 345899MTHFD1/2 Inhibitor -
HY-10446
PralatrexateDihydrofolate Reductasean Inhibitor -
HY-14518
AminopterinFolic Acid/DHFR Antagonist -
HY-10820R
Pemetrexed (Standard) -
HY-14530
PelitrexolGARFT Inhibitor
Biological Activity
Description
In Vitro
10-Formylfolic acid (80 nM; 2 min) potently inhibits purified rat liver dihydrofolate reductase with an IC50 of 80 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
10-Formylfolic acid (92 pmol/kg body weight; oral; single dose) is absorbed and metabolized in healthy Sprague Dawley rats in a manner quantitatively and qualitatively similar to folic acid, with 10.3% of the oral dose recovered as hepatic 5-methyltetrahydrofolic acid at 24 hours[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cobb × Cobb (male, day-old, folacin-deficient model)[1]
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Dosage:1.60 nmol/g of diet
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Administration:oral; ad libitum; 21 days
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Result:Achieved a mean weight gain of 523 g.
Achieved a mean feed consumption of 32.9 g per chick per day.
Achieved a mean plasma folacin concentration of 14.7 ng/mL.
Exhibited 97.5% folacin activity relative to folic acid based on plasma folacin dose-response curve analysis.
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Animal Model:Crl:CD(SD)BR Sprague Dawley (male, 500-600 g, normal healthy)[1]
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Dosage:92 pmol/kg body weight
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Administration:oral; single dose
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Result:Resulted in 10.3% of the oral dose recovered as hepatic 5-methyltetrahydrofolic acid 24 hours after dosing.
Produced a relative distribution of radiolabeled hepatic folates: 25% tetrahydrofolic acid, 63% 5-methyltetrahydrofolic acid, and 12% 5-formyltetrahydrofolic acid.
Chemical Information
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CAS No. 134-05-4
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Appearance Solid
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Molecular Weight 469.41
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Formula C20H19N7O7
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Color Light yellow to brown
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SMILES
O=C(O)[C@@H](NC(C1=CC=C(N(C=O)CC2=NC3=C(N=C2)N=C(N)NC3=O)C=C1)=O)CCC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
10-Formylfolic acid Related Classifications
HY-124350 Related Classifications
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