2',5'-Dideoxy-3'-ATP 

2',5'-Dideoxy-3'-ATP is an adenylate cyclase inhibitor. 2',5'-Dideoxy-3'-ATP exerts linear noncompetitive inhibition via binding to the distinct P-site. 2',5'-Dideoxy-3'-ATP binds and stabilizes the dimeric form of Mycobacterium avium adenylyl cyclase Ma1120 to inhibit activity^[1][2][3].

2',5'-Dideoxy-3'-ATP potently inhibits rat brain adenylyl cyclase with an IC₅₀ of 40 nM^[1].
2',5'-Dideoxy-3'-ATP inhibits purified bovine brain adenylyl cyclase with an IC₅₀ of 120 nM^[1].
2',5'-Dideoxy-3'-ATP directly inhibits purified recombinant Type I adenylyl cyclase (from Sf9 cells) with an IC₅₀ of 170 nM^[1].
2',5'-Dideoxy-3'-ATP (3 min) potently inhibits Forskolin (HY-15371)-stimulated activity of purified wild-type adenylyl cyclase (wt-AC) with a K_i of 200 nM in a fluorescence-based cyclase assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

475.18

C₁₀H₁₆N₅O₁₁P₃

162554-02-1

NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](OP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](C)O3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Désaubry L, et al. 2',5'-Dideoxyadenosine 3'-polyphosphates are potent inhibitors of adenylyl cyclases. J Biol Chem. 1996 Feb 2;271(5):2380-2.

  [2]. Vadakkadathmeethal K, et al. Fluorescence-based adenylyl cyclase assay adaptable to high throughput screening. Comb Chem High Throughput Screen. 2007;10(4):289-298.

  [3]. Barathy DV, et al. Autoinhibitory mechanism and activity-related structural changes in a mycobacterial adenylyl cyclase. J Struct Biol. 2015;190(3):304-313.