Copy Link Print Information

2′,7′-Dichlorofluorescein diacetate

(Synonyms: DCFH2-DA)
25 Cited Publications
Customer Review

Based on 25 publication(s) in Google Scholar

2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe. 2′,7′-Dichlorofluorescein diacetate can be used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon.

For research use only. We do not sell to patients.

  • Purity: 99.16%
  • CAS No.: 2044-85-1
  • Formula: C24H14Cl2O7
  • Molecular Weight:485.27
  • Storage:

    4°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • 2′,7′-Dichlorofluorescein diacetate Chemical Structure
    2′,7′-Dichlorofluorescein diacetate Chemical Structure
  • Experimental Validation Results for 2′,7′-Dichlorofluorescein diacetate
  • Experimental Validation Results for 2′,7′-Dichlorofluorescein diacetate
  • Experimental Validation Results for 2′,7′-Dichlorofluorescein diacetate
  • Experimental Validation Results for 2′,7′-Dichlorofluorescein diacetate
  • Experimental Validation Results for 2′,7′-Dichlorofluorescein diacetate
CAS No.: 2044-85-1
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Data Download
Other Forms of 2′,7′-Dichlorofluorescein diacetate:

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Chemical Information
  • Solvent & Solubility
  • Purity & Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) 2′,7′-Dichlorofluorescein diacetate

More
Customer Validation & Images
Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
Keywords
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00
Sign in to MedChemExpress Sign In
Register
Sign in to MedChemExpress
Register New Account

At least 6 characters

Personalize your experience, you are currently not signed in. For a better experience, please create an online profile, you can:

Obtain points redemption prizes
Check out faster
Add multiple shipping locations
View orders
Assay Types
  • 2′,7′-Dichlorofluorescein diacetate Chemical Structure
    2′,7′-Dichlorofluorescein diacetate Chemical Structure
    1/16
  • 1/16

    2′,7′-Dichlorofluorescein diacetate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct:146:157098. [Abstract]

    Customer Validation
     ROS levels in A549 cells after 4 h of Flp-A treatment were measured using a ROS detection probe (2′,7′-dichlorofluorescein diacetate (DCFH2-DA)) (scale bar = 100 μm).

  • 1/16

    2′,7′-Dichlorofluorescein diacetate purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 Oct 30:490:145079. [Abstract]

    Customer Validation
     The impact of RPPH-1 treatment on the activities of reactive oxygen species (ROS) in C. elegans. In this experiment, 2′,7′-dichlorofluorescein diacetate (DCFH2-DA, 10 mM; 1 h) was used to detect ROS.

  • 1/16

    2′,7′-Dichlorofluorescein diacetate purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Nov;36(45):e2409618. [Abstract]

    Customer Validation
     Intracellular ROS level of Hepa 1-6 cells after different treatments. This experiment utilized the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (10 μM; 25 min) probe to visualize and characterize the generation process of ROS.

  • 1/16

    2′,7′-Dichlorofluorescein diacetate purchased from MedChemExpress. Usage Cited in: Chin Med. 2024 Apr 8;19(1):59. [Abstract]

    Customer Validation
     H9C2 cells were stained with 2′,7′-Dichlorofluorescein diacetate (DCFH2-DA, 1 µM) to detect the ROS level.

  • 1/16

    2′,7′-Dichlorofluorescein diacetate purchased from MedChemExpress. Usage Cited in: Food Hydrocoll. 93 (2019) 261-269.

    Customer Validation
     Images of control group (Control), H2O2 treated group, H2O2 treated and then digestion fluid of zein particle encapsulated resveratrol treated group (ZP), digestion fluid of free resveratrol group treated group (Free Res), and digestion fluid of physical mixture treated group (PM). The higher the fluorescence intensity, the higher the ROS level. The digestion fluid samples had been subjected to 120 min stimulated gastric digestion and 240 min small intestinal digestion prior to analysis.
    H2O2 was used to cause oxidative damage to the cells, and 2′,7′-Dichlorofluorescein diacetate (DCFH-DA) was used as a fluorescence probe to monitor the intracellular ROS level. A very weak fluorescence signal was observed for the control, which indicated that there was little ROS in the HepG2 cells themselves.

  • 2′,7′-Dichlorofluorescein diacetate