2-Azidoethyl β-D-glucopyranoside
2-Azidoethyl β-D-Glucopyranoside is a biochemical reagent that can be used as a biological material or organic compound for life science related research. 2-Azidoethyl β-D-glucopyranoside is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- CAS No.: 165331-08-8
- Formula: C8H15N3O6
- Molecular Weight:249.22
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
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Biological Activity
Description
Chemical Information
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CAS No. 165331-08-8
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Molecular Weight 249.22
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Formula C8H15N3O6
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SMILES
[N-]=[N+]=NCCO[C@@H]1O[C@@H]([C@H]([C@@H]([C@H]1O)O)O)CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2-Azidoethyl β-D-glucopyranoside Related Classifications
HY-W145563 Related Classifications
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