2-Deacetyl-4-tigloylchamissionolide
2-Deacetyl-4-tigloylchamissionolide is a heliangolide-type sesquiterpene lactone, a derivative of Chamissonolide (HY-N19727), and a cytotoxic agent. 2-Deacetyl-4-tigloylchamissionolide exerts cytotoxicity via alkylating biological nucleophiles, targeting free thiol groups in proteins to interfere with their functions. 2-Deacetyl-4-tigloylchamissionolide exhibits different chromatographic behaviors in TLC and HPLC. 2-Deacetyl-4-tigloylchamissionolide can be used for the research of Ehrlich ascites carcinoma.
For research use only. We do not sell to patients.
- CAS No.: 173401-52-0
- Formula: C20H28O6
- Molecular Weight:364.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
2-Deacetyl-4-tigloylchamissionolide (compound 20) (2 h; 72 h) exhibits cytotoxicity against murine EN2 cloned Ehrlich ascites tumor cells, with an IC50 of 116 μM after 2 h incubation and 10.4 μM after 72 h incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 173401-52-0
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Molecular Weight 364.43
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Formula C20H28O6
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SMILES
C[C@@]12[C@@H](C[C@@H]([C@@]1([H])[C@@H](C[C@]3([H])[C@](C(C(O3)=O)=C)([H])[C@@H]2O)C)O)OC(/C(C)=C/C)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2-Deacetyl-4-tigloylchamissionolide Related Classifications
HY-N19808 Related Classifications
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Keywords
- 2-Deacetyl-4-tigloylchamissionolide
- 173401-52-0
- Drug Derivative
- chamissonolide derivative
- helenanolide-type sesquiterpene lactone
- Arnica species
- free sulfhydryl groups
- Michael-type addition
- ehrlich ascites tumor
- murine EN2 cloned Ehrlich ascites tumor cells
- cytotoxic agent
- biological nucleophiles
- Inhibitor
- inhibitor
- inhibit