2-O-(α-D-Glucopyranosyl)glycerol (55% w/w in water)
Based on 1 Customer Validation
2-O-(α-D-Glucopyranosyl) glycerol (55% w/w in water) (Glucosylglycerol) is a glycerol glycoside and compatible solute. 2-O-(α-D-Glucopyranosyl) glycerol is isolable from the marine cyanobacterium Oscillatoria sp. 2-O-(α-D-Glucopyranosyl) glycerol (55% w/w in water) acts as a salt stress protectant in Synechocystis sp. PCC 6803 ΔggpS cells. It enhances salt tolerance of Synechocystis sp. PCC 6803 ΔggpS cells and maintains cell division under salt stress.
For research use only. We do not sell to patients.
- Purity : 99.79%
- CAS No.: 22160-26-5
- Formula: C9H18O8
- Molecular Weight:254.23
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Storage:
Solution, -20°C, 2 years
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Glucosylglycerol (1 mM; up to 5 days) reverses cell division arrest, cell enlargement and lysis induced by 450 mM NaCl in Synechocystis sp. PCC 6803 ΔggpS mutant cells. It exerts the strongest efficacy when added within 2 days after salt stress, and restores normal cell size and ultrastructure[2].
Glucosylglycerol (1 mM; up to 5 days) restores the growth of Synechocystis sp. PCC 6803 ΔggpS mutant cells under 450 mM NaCl salt stress; the growth restoration effect is most potent when the substance is added 1 day after the initiation of stress[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 22160-26-5
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Appearance Liquid
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Molecular Weight 254.23
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Formula C9H18O8
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Color Colorless to light yellow
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SMILES
C(C(CO)O[C@@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O)O
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Synonyms
Glucosylglycerol
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Structure Classification
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Initial Source
Oscillatoria sp.
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Solvent & Solubility
In Vitro:
H2O : ≥ 175 mg/mL (688.35 mM)
* "≥" means soluble, but saturation unknown.
Protocols
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CFSE Dye Dilution Proliferation Assay
The CFSE (carboxyfluorescein diacetate succinimidyl ester) dye dilution proliferation assay is based on the covalent labeling of intracellular proteins by a cell-permeant fluorescent dye that becomes fluorescent upon intracellular ester cleavage and then is stably retained within cells. As labeled cells divide, the dye is partitioned equally between daughter cells, resulting in a stepwise halving of fluorescence intensity that can be quantified by flow cytometry to determine the number of cell divisions undergone by each cell population. This fluorescence dilution approach enables quantitative tracking of lymphocyte proliferation at the single-cell level over multiple rounds of division. CFSE-based proliferation analysis has been widely applied to measure antigen-driven lymphocyte expansion in vitro, where discrete fluorescence peaks correspond to successive cell divisions and allow reconstruction of proliferative history within heterogeneous populations.
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Dye-dilution cell tracking and proliferation staining
Dye-dilution cell tracking assays quantify cell proliferation by covalently labeling intracellular proteins with a stable fluorescent dye that is equally partitioned between daughter cells during mitosis, resulting in stepwise halving of fluorescence intensity with each cell division as measured by flow cytometry histograms. Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a prototypical dye that diffuses into cells, is enzymatically converted into a fluorescent compound, and then covalently binds intracellular amine groups, producing long-lived fluorescence suitable for tracking multiple rounds of division in vitro and in vivo. Successive generations of dividing cells form discrete peaks of decreasing fluorescence intensity, enabling estimation of proliferation history, precursor frequency, and division index within heterogeneous populations. Alternative dyes such as CellTrace Violet (CTV) and far-red membrane dyes (e. g. , PKH26) follow the same dilution principle but differ
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.9334 mL | 19.6672 mL | 39.3345 mL | 98.3362 mL |
| 5 mM | 0.7867 mL | 3.9334 mL | 7.8669 mL | 19.6672 mL | |
| 10 mM | 0.3933 mL | 1.9667 mL | 3.9334 mL | 9.8336 mL | |
| 15 mM | 0.2622 mL | 1.3111 mL | 2.6223 mL | 6.5557 mL | |
| 20 mM | 0.1967 mL | 0.9834 mL | 1.9667 mL | 4.9168 mL | |
| 25 mM | 0.1573 mL | 0.7867 mL | 1.5734 mL | 3.9334 mL | |
| 30 mM | 0.1311 mL | 0.6556 mL | 1.3111 mL | 3.2779 mL | |
| 40 mM | 0.0983 mL | 0.4917 mL | 0.9834 mL | 2.4584 mL | |
| 50 mM | 0.0787 mL | 0.3933 mL | 0.7867 mL | 1.9667 mL | |
| 60 mM | 0.0656 mL | 0.3278 mL | 0.6556 mL | 1.6389 mL | |
| 80 mM | 0.0492 mL | 0.2458 mL | 0.4917 mL | 1.2292 mL | |
| 100 mM | 0.0393 mL | 0.1967 mL | 0.3933 mL | 0.9834 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2-O-(α-D-Glucopyranosyl)glycerol (55% w/w in water) Related Classifications
HY-W353469 Related Classifications
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Keywords
- 2-O-(α-D-Glucopyranosyl)glycerol (55% w/w in water)
- 22160-26-5
- Glucosylglycerol
- Biochemical Assay Reagents
- cell division arrest
- ultrastructure
- Synechocystis sp. PCC 6803
- salt stress
- ABC-type transport system
- cell size
- Oscillatoria sp.
- wild-type Synechocystis sp. PCC 6803 cells
- cell division
- Synechocystis sp. PCC 6803 ΔggpS cells
- Inhibitor
- inhibitor
- inhibit